ADHESIVE PROPERTIES AND SENSITIVITY TO ANTIFUNGAL DRUGS OF CANDIDA ALBICANS, WHICH ARE RELEASED FROM PATIENTS OF GASTROENTEROLOGICAL PROFILE WITH CANDIDIASIS OF THE MUCOSA OF THE UPPER PART OF THE DIGESTIVE TRACT

Inessa Kushnirenko

Abstract


Candida albicans were released from 146 loci from 89 patients in order to perform the investigation. The fourth stage of contamination by Candida albicans in the scrape from the tongue and candidiasis of the esophagus or /and the stomach during microbiologic investigations of the biopsy materials were the main criterion for this research. HIV-infected patients and patients in whom antibacterial therapy was done less than in four weeks were excluded from the investigation. Microorganisms were taken from the next biopsy materials: oropharyngeal area included 81, esophagus contained 30, stomach included 24 in general (the body of the stomach contained 14 and antrum included 10, gastric juice included 11. Adhesion evaluation was done by microtechnique in U-shaped microplates with formalized erythrocytes 0(І), Rh +. According to the results of the investigation, the released fungi in 51,37 % of cases were characterized as nonadherent ones, in 28,77 % of cases it was defined as low level of adhesiveness and only in 17,12 % of cases it was determined as average and high level of the adhesiveness. Adhesiveness increase was not associated with the increase of the contamination of the oral cavity and with the severity of the damage of the mucosa of the esophagus or /and the stomach. In 97,8 % of cases, strains of Candida albicans were sensitive to fluconazole, in 75,6 % of cases, strains were sensitive to itraconasole and in 24,4 % of cases they were determined as moderately sensitive and resistant that initiates fluconazole as the drug of the choice for the treatment of the candidiasis of the mucosa of the upper part of the digestive tract. So, received data determines the necessity to detect factors of dysfunction of antifungal resistance of the mucosa that detects the development of its candidal damage.

Keywords


candidiasis; mucosa; adhesion; sensitivity; digestive tract

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References


Yang, Yu.-L. (2003). Virulence factors of Candida species. J. Microbiol Immunol Infect., 36, 223–228.

Mohandas, V., Ballal, M. (2011). Distribution of Candida Species in different clinical samples and their virulence: Biofilm formation, proteinase and phospholipase production: A study on hospitalized patients in Southern India. Journal of Global Infectious Diseases, 3 (1), 4. doi: 10.4103/0974-777x.77288

Imron Nasution, A. (2013). Virulence Factor and Pathogenicity of Candida albicans in Oral Candidiasis. World Journal of Dentistry, 4, 267–271. doi: 10.5005/jp-journals-10015-1243

Hebecker, B., Naglik, J. R., Hube, B., Jacobsen, I. D. (2014). Pathogenicity mechanisms and host response during oral Candida albicans infections. Expert Review of Anti-Infective Therapy, 12 (7), 867–879. doi: 10.1586/14787210.2014.916210

Zhu, W., Filler, S. G. (2010). Interactions ofCandida albicanswith epithelial cells. Cellular Microbiology, 12 (3), 273–282. doi: 10.1111/j.1462-5822.2009.01412.x

Kumamoto, C. A. (2011). Inflammation and gastrointestinal Candida colonization. Current Opinion in Microbiology, 14 (4), 386–391. doi: 10.1016/j.mib.2011.07.015

Glittenberg, M. T., Kounatidis, I., Christensen, D., Kostov, M., Kimber, S., Roberts, I., Ligoxygakis, P. (2011). Pathogen and host factors are needed to provoke a systemic host response to gastrointestinal infection of Drosophila larvae by Candida albicans. Disease Models & Mechanisms, 4 (4), 515–525. doi: 10.1242/dmm.006627

Gow, N. A., Hube, B. (2012). Importance of the Candida albicans cell wall during commensalism and infection. Current Opinion in Microbiology, 15 (4), 406–412. doi: 10.1016/j.mib.2012.04.005

Eggimann, P., Pittet, D. (2014). Candida colonization index and subsequent infection in critically ill surgical patients: 20 years later. Intensive Care Medicine, 40 (10), 1429–1448. doi: 10.1007/s00134-014-3355-z

Sampaio, P., Santos, M., Correia, A., Amaral, F. E., Chavéz-Galarza, J., Costa-de-Oliveira, S. et. al. (2010). Virulence Attenuation of Candida albicans Genetic Variants Isolated from a Patient with a Recurrent Bloodstream Infection. PLoS ONE, 5 (4), e10155. doi: 10.1371/journal.pone.0010155

Bernhardt, J., Herman, D., Sheridan, M., Calderone, R. (2001). Adherence and Invasion Studies of Candida albicans Strains, Using In Vitro Models of Esophageal Candidiasis . The Journal of Infectious Diseases, 184 (9), 1170–1175. doi: 10.1086/323807

Biasoli, M. S., Tosello, M. E., Magaro, H. M. (2002). Adherence of Candida strains isolated from the human gastrointestinal tract – Adharenz von Candida-Isolaten aus dem menschlichen Gastrointestinaltrakt. Mycoses, 45 (11-12), 465–469. doi: 10.1046/j.1439-0507.2002.d01-123.x

Mayer, F. L., Wilson, D., Hube, B. (2013). Candida albicans pathogenicity mechanisms. Virulence, 4 (2), 119–128. doi: 10.4161/viru.22913

Gow, N. A. R., van de Veerdonk, F. L., Brown, A. J. P., Netea, M. G. (2011). Candida albicans morphogenesis and host defence: discriminating invasion from colonization. Nature Reviews Microbiology. doi: 10.1038/nrmicro2711

Williams, D. W., Jordan, R. P. C., Wei, X.-Q., Alves, C. T., Wise, M. P., Wilson, M. J., Lewis, M. A. O. (2013). Interactions of Candida albicans with host epithelial surfaces . Journal of Oral Microbiology, 5 (0). doi: 10.3402/jom.v5i0.22434

Grubb, S. E. W., Murdoch, C., Sudbery, P. E., Saville, S. P., Lopez-Ribot, J. L., Thornhill, M. H. (2009). Adhesion of Candida albicans to Endothelial Cells under Physiological Conditions of Flow. Infection and Immunity, 77 (9), 3872–3878. doi: 10.1128/iai.00518-09

Rast, T. J., Kullas, A. L., Southern, P. J., Davis, D. A. (2016). Human Epithelial Cells Discriminate between Commensal and Pathogenic Interactions with Candida albicans. PLoS ONE, 11 (4), e0153165. doi: 10.1371/journal.pone.0153165

Vautier, S., Drummond, R. A., Chen, K., Murray, G. I., Kadosh, D., Brown, A. J. P. et. al. (2014). Candida albicanscolonization and dissemination from the murine gastrointestinal tract: the influence of morphology and Th17 immunity. Cellular Microbiology, 17 (4), 445–450. doi: 10.1111/cmi.12388

Kerimov, E. А. (2011). Issledovanie vzaimosvyazi kolichestvennoy vysevaemosti Candida spp. S faktor ami patogennosti griba u bol’nyh kandidozom zheludochno-kischechnogo trakta [Study of the interrelationship of quantitative inoculation Candida spp. with fingi pathogenicity factors in patients with candidiasis of the gastrointestinal tract]. Ukrainskiy zhurnal klinichnoi ta laboratornoi mediciny, 6 (1), 103–108.

Lisovskaya, S. А., Haldeeva, E. V., Gluschko, N. I., Parschakov, V. R. (2015). Analiz adgezivnoy aktivnosti klinicheskih schtammov Candida albicans, dydelennyh s kozhi bolnyh raznyh nozologicheskih grupp [Analysis of the adhesive activity of Candida albicans, isolated from skin of patients with different nosological groups]. Problemy medizinskoy mikologii, 17 (4), 66–68.

Polishuk, O. I., V’yalyh, Zh. Е., Yanovs’ka, V. V., Pokas, O. V., Makuschenko, O. S. (2008). Zastosuvannya micrometodu viznachennya adgezivnoi aktivnosti yak faktoru patogennosti microorganizmiv [Apply adhesive micromethod determining activity as a factor of pathogenicity of microorganisms]. Information paper № 30. Ukrmedpatentinform MOZ of Ukraine, 3.




DOI: http://dx.doi.org/10.21303/2504-5679.2016.00221

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Copyright (c) 2016 Inessa Kushnirenko

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