CHARACTERISTIC OF INDIVIDUAL INDICATORS OF ENDOGENOUS INTOXICATION IN THE EXPERIMENTAL ASTHMA DYNAMICS AND THEIR CORRECTION BY THIOTRIASOLIN

  • Marta Kolishetska Danylo Halytskiy Lviv National Medical University, Ukraine
Keywords: bronchial asthma, endogenous intoxication, middle mass molecules, erythrocyte intoxication index, thiotriasolin

Abstract

The aim of this work was to find out the specifics of changes in endogenous intoxication, middle mass molecules (MMM) and erythrocyte intoxication index (EII) in the blood of animals for experimental asthma in different periods of its development and to correct them by thiotriazolin.

Material and methods. Researches were carried out on 72 guinea-pigs (males), which were divided into 6 groups for 12 animals in each of them.  The last sixth group included animals that were treated by thiotriazolin. The drug thiotriazolin rate of 100 mg / kg intramuscularly since 23rd day of the experiment for 10 days was administered in the animals of sixth group.  

Experimental model of bronchial asthma was restored on guinea-pigs by the V.I. Babych method (1979). In all groups of animals there were determined the middle mass molecules concentration in blood in the wave of 254 nm by I.A.Volchehorskiy, D.A.Dyatlova, E.I. Lvovska and others methods and EII by V.K. Kazymyrko V.I. Maltsev methods. Numerical results were adapted with static method using Student’s criteria.

Results of the researchThe highest level of endogenous intoxication in the blood was in the fifth group of guinea pigs with asthma (33rd day), indicating the direct dependence of the antigenic effect on their severity. Using of the thiotriazolin within 10 days (from 23rd to 33rd day) caused the decrease of the MMM254 concentration and EII in serum compared with a group of guinea pigs, that were not entered the medicine. It approved its treating effect.

ConclusionsSo, the research of middle mass molecules and erythrocyte intoxication index indicators in different periods of experimental asthma showed their gradual increase in serum of guinea pigs with the greatest severity in 33rd day of the experiment. It might indicate the development of endogenous intoxication animals. But the using of thiotriazolin caused the decreasing of these indicators. That gives a reason to state its positive effect on some markers of endogenous intoxication and feasibility of further researches.

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Author Biography

Marta Kolishetska, Danylo Halytskiy Lviv National Medical University

Department of pathophisiology

References

Alifanova, S. V. (2013). Risk Factors Of Bronchial Asthma in Children. Actual questions in pharmaceutical and medical science, 3 (13), 4–7.

Regeda, M. S., Furdychko, L. O., Kolishetska, M. A. (2012). Bronchial Asthma. Lviv, 147.

Regeda, M. S., Pogoretska, Y. O. (2013). Bronchial Asthma:changes of separated indicators of endogenous intoxication and their correction by thiotriazoline. Actual problems of transport medicine, 3 (33), 111–115.

Global strategy for asthma management and prevention. National institutes of health. National Heart, Lung and Blood Institute. Revised 2014. Available at: http://www.ginasthma.org

Hedlin, G., Konradsen, J., Bush, A. (2012). An update on paediatric asthma. European Respiratory Review, 21 (125), 175–185. doi: 10.1183/09059180.00003212

Shmoilov, D. K., Karimov, I. Z., Odynets, T. N. (2012). Pathogenic role of endogenous intoxication. Lab. Diagnostic, 2, 65–69.

Kit, B. K., Simon, A. E., Ogden, C. L., Akinbami, L. J. (2011). Trends in Preventive Asthma Medication Use Among Children and Adolescents, 1988–2008. PEDIATRICS, 129 (1), 62–69. doi: 10.1542/peds.2011-1513

Waserman, S., Nair, P., Snider, D., Conway, M., Jayaram, L., McCleary, L. M. et. al. (2012). Local and systemic immunological parameters associated with remission of asthma symptoms in children. Allergy, Asthma & Clinical Immunology, 8 (1), 16. doi: 10.1186/1710-1492-8-16

Pike, K. C., Crozier, S. R., Lucas, J. S. A., Inskip, H. M., Robinson, S. et. al. (2010). Patterns of fetal and infant growth are related to atopy and wheezing disorders at age 3 years. Thorax, 65 (12), 1099–1106. doi: 10.1136/thx.2010.134742

Uddin, M., Nong, G., Ward, J., Seumois, G., Prince, L. R., Wilson, S. J. et. al. (2010). Prosurvival activity for airway neutrophils in severe asthma. Thorax, 65 (8), 684–689. doi: 10.1136/thx.2009.120741

Cornwell, W., Kim, V., Song, C., Rogers, T. (2010). Pathogenesis of Inflammation and Repair in Advanced COPD. Seminars in Respiratory and Critical Care Medicine, 31 (03), 257–266. doi: 10.1055/s-0030-1254066

Gernez, Y., Tirouvanziam, R., Chanez, P. (2010). Neutrophils in chronic inflammatory airway diseases: can we target them and how? European Respiratory Journal, 35 (3), 467–469. doi: 10.1183/09031936.00186109

Agache, I., Akdis, C., Jutel, M., Virchow, J. C. (2012). Untangling asthma phenotypes and endotypes. Allergy, 67 (7), 835–846. doi: 10.1111/j.1398-9995.2012.02832.x

Umanec, T. R. (2011). Clinic-anamnestic features of fenotypes of bronchial asthma for children. Perinatology and paediatrics, 2 (46), 69–71.

Hesselmar, B., Enelund, A.-C., Eriksson, B., Padyukov, L., Hanson, L. A., Aberg, N. (2012). The Heterogeneity of Asthma Phenotypes in Children and Young Adults. Journal of Allergy, 2012, 1–6. doi: 10.1155/2012/163089

Opanasenko, G. V., Potter, О. О., Phrancuzova, S. B., Mankovska, I. N. (2012). Influence of the thiotriasoline on the state of pro- and antioxidant balance in soft fabrics of paradont at the terms of chronic stress. Tavricheskiy medico-biological vistnyk, 15 (3), 246–249.

Volchegorskiy, I. A., Diatlov, D. A., Lvovskaya, E. I. et. al. (1997). Middle molecules" as probable regulators of erythrone system in skiers. Lab. Diagnostic, 1, 11–16.

Kazymyrko, V. K., Maltsev, V. I. (2007). Antioxidant system and its function in a human body. Ukrainian Health, 5, 15–24.

Аntypkin, Y. G. et. al. (2010). The clinical discipling from diagnostics and treatment of bronchial asthma in children. Health of Ukraine, 3 (14), 39–41.

Bezrukov, L. O., Bezrukov, O. K., Ivanov, L. A. et. al. (2011). The chosen questions of diagnostics and treatment of bronchial asthma in children. Chernivtsi: City, 203.


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Published
2016-12-14
How to Cite
Kolishetska, M. (2016). CHARACTERISTIC OF INDIVIDUAL INDICATORS OF ENDOGENOUS INTOXICATION IN THE EXPERIMENTAL ASTHMA DYNAMICS AND THEIR CORRECTION BY THIOTRIASOLIN. EUREKA: Health Sciences, (6), 52-56. https://doi.org/10.21303/2504-5679.2016.00248
Section
Medicine and Dentistry