THE LYMPH NODES IN RATS WITH EXPERIMENTAL TYPE 1 DIABETES MELLITUS (DM-1)

Margaryta Khomenko, Iurii Gavrylenko

Abstract


The aim is to study morphofunctional structure of NALT (nasal associated lymphoid tissue) and visceral lymph nodes of rats with experimental type 1 diabetes mellitus (DM-1) and to define the effectiveness of the treatment with herbal drug “Imupret”.

Materials and methods: The experiment involved 20 animals, divided into 4 groups: the 1st group was the control group of healthy rats, the 2nd group was the control group subject to prophylactic treatment with “Imupret, the 3rd group included rats with experimental diabetes, and the 4th group included rats with experimental diabetes subject to treatment with “Imupret. Functional changes in immune organs were evaluated by the results of morphometric analysis; morphological pattern was evaluated by histostructural changes.

Results. The research revealed that under conditions of diabetes mellitus type 1, the volume, area and density of the lymphoid tissue decreased, and only its "fine" cell was detected. The paper demonstrates the development of relative immune deficiency in immunocompetent organs in rats with diabetes mellitus type 1. The use of drug "Imupret" demonstrated its immunomodulatory function, which is especially important in terms of immunosuppression in patient with DM-1.

Conclusion. The received results are of a great clinical significance, and show the necessity of early prevention and treatment of immunity disorders under conditions of diabetes mellitus type 1. 


Keywords


diabetes mellitus type 1; visceral lymph nodes; NALT; immunity histostructural changes; morphometric analysis

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DOI: http://dx.doi.org/10.21303/2504-5679.2017.00297

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Copyright (c) 2017 Margaryta Khomenko

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ISSN 2504-5679 (Online), ISSN 2504-5660 (Print)