RESEARCH OF CERTAIN PATHOGENIC CHARACTERISTICS OF CLINICAL ISOLATES OF STAPHYLOCOCCI OF SKIN BIOME

Yanina Kutasevych, Svetlana Dzhoraeva, Valentina Goncharenko, Yuliia Shcherbakova, Viktoriya Mangusheva, Nataliya Sobol, Helen Shchegoleva

Abstract


A serious problem in patients with atopic dermatitis (AD) is the frequent attachment of a secondary skin infection. Among the microbes colonizing the skin of patients suffering from AD, S. aureus takes the lead. According to different authors, from the skin of 80–95 % of patients are sown Staphylococcus aureus. The survival of bacteria in a biotope is promoted by the persistent properties of microorganisms.

Aim of the research: to determine the adhesive properties and antilysozyme activity of clinical strains of staphylococci isolated from the skin of patients with allergic dermatosis.

The study included 50 patients with atopic dermatitis and 20 practically healthy individuals, from which 140 laboratory strains of staphylococci were isolated: 101 strains from patients with AD and 39 control strains. Bacteriological studies to isolate microorganisms and determine a number of pathogenic characteristics were carried out using the methods of classical bacteriology.

The severity of antilysozyme activity (ALA) and adhesive properties of strains isolated from affected areas of the skin was significantly higher than in cultures isolated from intact skin areas, both qualitatively and quantitatively. The obtained data made it possible to assume a certain complicating role of these factors on the course of AD.


Keywords


clinical strains of staphylococci; antilysozyme activity; adhesive properties

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References


Ponomarenko, S. V. (2013). Mikrobiologicheskie aspekty stafilokokkovoy infektsii na sovremennom etape (obzor literatury). Annals of Mechnikov Institute, 3, 13–17. Available at: http://www.imiamn.org.ua/journal/3_2013/PDF/4.pdf

Belousova, T. A., Goryachkina, M. A., Katranova, D. G. (2013). Osobennosti microbiotsenoza kozhi u bolnih allergodermatozami: problema vibora napuzhnoy terapii. Clinicheskaya dermatologiya i venerologiya, 11 (3), 107–112.

Kramar, L. V., Zhadchenko, U. V., Khlynina, U. O., Rodionova, N. V. (2012). Fenotipicheskie harakteristiki populyatsii S. aureus, vydelennyh ot razlichnyh kategoriy nositeley. Fundamentalnye issledovaniya, 4 (2), 295–298.

Zecconi, A., Scali, F. (2013). Staphylococcus aureus virulence factors in evasion from innate immune defenses in human and animal diseases. Immunology Letters, 150 (1-2), 12–22. doi: 10.1016/j.imlet.2013.01.004

Buharin, O. V. (2008). Persistentsiya bakterialnyh patogenov kak fiziologicheskiy fenomen. Vestnik Moskovskogo Universiteta. Seriya 16. Biologiya, 1, 6–13.

Mikhailova, L. V., Kramar, V. O., Savchenko, T. N., Klimovа, T. N. (2010). Factory sposobstvuyushchie persistentsii uslovno-patogennyh microorganizmov. Vestnik VolGMU, 4 (36), 76–79.

Grebennikova, V. V., Bakscheeva, S. S., Sergeeva, I. V. (2016). Mehanizmy formirovaniya bakterialnogo persistirovaniya microorganizmov, vydelennyh u detey, prozhivaushchih v raznyh ekologicheskih usloviyah. Sovremennye problemy nauki i obrazovaniya, 6. Available at: https://www.science-education.ru/ru/article/view?id=25667

Paharik, A. E., Horswill, A. R. (2016). The Staphylococcal biofilm: adhesins, regulation, and host response. Virulence Mechanisms of Bacterial Pathogens, 529–566. doi: 10.1128/microbiolspec.vmbf-0022-2015

Suharyev, A. V., Gutka, V. O., Patrushev, A. V., Bondar, O. I., Nazarov, R. N. (2012). Kombinirovannaya naruzhnaya terapiya atopicheskogo dermatita, oslozhnyennogo vtorichnoy infektsiey. Vestnik dermatologii i venerologii, 5, 113–118.

Popov, I. V. (2014). Osobennosti microbiotsenoza kozhi pri atopicheskom dermatite. Vestnik poslediplomnogo meditsinskogo obrazovaniya, 2, 58–62.

Arya, R., Princy, S. A. (2015). Exploration of Modulated Genetic Circuits Governing Virulence Determinants in Staphylococcus aureus. Indian Journal of Microbiology, 56 (1), 19–27. doi: 10.1007/s12088-015-0555-3

Batyrshina, S. V., Chaertdinova, L. A., Chalilova, R. G. et. al. (2013). Microbiotsenoz kozhi u bolnyh atopicheskim dermatitom i ego korrektsiya. Prakticheskaya meditsina, 1-4 (73), 33–37.

Gayrabekov, P. H., Gayrabekova, P. H., Gubhanova, S. A., Turlova, F. S., Umieva, Z. E. (2016). Antilizotsimnaya aktivnost nekotoryh enterobakteriy. Mezhdunarodniy zhurnal prikladnyh i fundamentalnyh issledovaniy, 7 (1), 63–64.

Shimada, T., Park, B. G., Wolf, A. J., Brikos, C., Goodridge, H. S., Becker, C. A. et. al. (2010). Staphylococcus aureus Evades Lysozyme-Based Peptidoglycan Digestion that Links Phagocytosis, Inflammasome Activation, and IL-1β Secretion. Cell Host & Microbe, 7 (1), 38–49. doi: 10.1016/j.chom.2009.12.008

Findik, B. T., Say, R., Unluer, O. B., Demirel, R., Ersoz, A. (2016). Lysozyme Cross-Linked Antibodious Destroyers Against Staphylococcus аureus. Hacettepe Journal of Biology and Chemistry, 44 (4), 477–486.

Bera, A., Herbert, S., Jakob, A., Vollmer, W., Gotz, F. (2005). Why are pathogenic staphylococci so lysozyme resistant? The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus. Molecular Microbiology, 55 (3), 778–787. doi: 10.1111/j.1365-2958.2004.04446.x

Kong, C., Chee, C.-F., Richter, K., Thomas, N., Abd. Rahman, N., Nathan, S. (2018). Suppression of Staphylococcus aureus biofilm formation and virulence by a benzimidazole derivative, UM-C162. Scientific Reports, 8 (1). doi: 10.1038/s41598-018-21141-2

Ob unifikatsii mikrobiologicheskih (bakteriologicheskih) metodov issledovaniya, primenyaemih v kliniko-diagnosticheskih laboratoriyah lechebno-profilakticheskih uchrezhdeniy (1985). Ministerstvo zdravookhraneniya SSSR, No. 535. Available at: http://www.alppp.ru/law/zdravoohranenie--fizicheskaja-kultura-i-sport--turizm/zdravoohranenie/64/prikaz-minzdrava-sssr-ot-22-04-1985--535.html

Baron, E. J., Miller, J. M., Weinstein, M. P., Richter, S. S., Gilligan, P. H., Thomson, R. B. et. al. (2013). A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2013 Recommendations by the Infectious Diseases Society of America (IDSA) and the American Society for Microbiology (ASM)a. Clinical Infectious Diseases, 57 (4), 22–121. doi: 10.1093/cid/cit278

Brilis, V. I., Brilene, T. A., Lentsner, Kh. P., Lentsner, A. A. (1986). Metodika izucheniya adgezivnogo protsessa microorganizmov. Laboratornoe delo, 4, 210–212.

Buharin, О. V., Valyshev, A. V., Elagina, E. E. (2000). Antilizotsimnaya aktivnost anaerobnyh bakteriy fekalnoy mikroflory cheloveka. Zhurnal microbiologii, epidemiologii i immunologii, 5, 20–22.

Falova, O. E. (2011). Vzaimosvyaz i stepen vurazhennosti adgezivnoy sposobnosti i antilizotsimnoy aktivnosti stafilikokkov, vydelennyh s kozhi ludey, stradaushchih hronicheskimi dermatozami. Vestnik Tomskogo Gosudarstvennogo Universiteta, 349, 188–189.




DOI: http://dx.doi.org/10.21303/2504-5679.2018.00585

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Copyright (c) 2018 Yanina Kutasevych, Svetlana Dzhoraeva, Valentina Goncharenko, Yuliia Shcherbakova, Viktoriya Mangusheva, Nataliya Sobol, Helen Shchegoleva

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