INVESTIGATION OF THE CHEMICAL COMPOSITION AND EFFICIENCY OF SEED OIL OF SPINDLE TREE (EUONYMUS EUROPAEA L.) ON THE MODEL OF NON-ALLERGIC DERMATITIS
Abstract
The aim of the research was to study the chemical composition and effectiveness of the seed oil of Spindle tree (Euonymus europaea L.) on the non-allergic contact dermatitis model.
The oil was obtained by the extraction by petroleum ether from the seeds. The analysis of fatty acids and determination of their quantitative content was carried out using gas chromatography. Determination of carotenoids and tocopherols content in fatty oils was carried out after chromatography on a silica gel column. Investigation of anti-inflammatory action of the Spindle tree seed oil on white rats was carried. Determination of biochemical parameters of blood plasma was performed on the semi-automatic biochemical analyzer BS3000M (Poland).
The yield of oil was 20–28 % of the weight of the seeds. Nine fatty acids were identified in the oil by the gas chromatography, among which 4 are unsaturated (oleic, palmitoleic, linoleic and linolenic), which together make up 87.79 % of all fatty acids from this oil. Spindle tree oil contains 26±5 mg – % carotenoids and 40±5 mg – % tocopherols, which were separated by chromatography on a silica gel column. There were no symptoms of intoxication with the introduction of Spindle tree oil in the stomach of rats and it can be classified in the 4 the grade of danger in accordance with GOST 12.1.005-88.
The anti-inflammatory activity of Spindle tree oil in comparison with the oil of Sea buckthorn on the model of non-allergic contact dermatitis was weaker, but sufficient to recommend it for the treatment of skin diseases in ointments.
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References
Johansen, J. D., Aalto-Korte, K., Agner, T., Andersen, K. E., Bircher, A., Bruze, M. et. al. (2015). European Society of Contact Dermatitis guideline for diagnostic patch testing – recommendations on best practice. Contact Dermatitis, 73 (4), 195–221. doi: 10.1111/cod.12432
Semenov, D. V. (2014). Study of Pharmacological Effect of Ointment with Aronia Oil of Blackheads. Ukrainian Biopharmaceutical Journal, 2, 31
Konovalova, O. Yu., Tumanov, V. A., Mitchenko, F. A. et. al. (2011). Poisonous Plants. PE "Wandering MX", 494.
Thomas, P. A., El-Barghathi, M., Polwart, A. (2010). Biological Flora of the British Isles: Euonymus europaeus L. Journal of Ecology, 99 (1), 345–365. doi: 10.1111/j.1365-2745.2010.01760.x
Kumarasamy, Y., Cox, P. J., Jaspars, M., Nahar, L., Wilcock, C. C., Sarker, S. D. (2003). Biological activity of Euonymus europaeus. Fitoterapia, 74 (3), 305–307. doi: 10.1016/s0367-326x(03)00022-4
Wanner, J., Tabanca, N., Zehl, M., Jirovetz, L., Schmidt, E., Patschka, A. et. al. (2015). Investigations into the Chemistry and Insecticidal Activity of Euonymus europaeus Seed Oil and Methanol Extract. Current Bioactive Compounds, 11 (1), 13–22. doi: 10.2174/157340721101150804142925
Zhu, J.-X., Qin, J.-J., Chang, R.-J., Zeng, Q., Cheng, X.-R., Zhang, F. et. al. (2012). Chemical Constituents of Plants from the Genus Euonymus. Chemistry & Biodiversity, 9 (6), 1055–1076. doi: 10.1002/cbdv.201100170
State Pharmacopoeia of Ukraine (2001). Kharkiv: RIREG, 556.
Grob, R. L., Barry, E. F. (2004). Modern Practice of Gas Chromatography. John Wiley & Sons, 1064. doi: 10.1002/0471651141
Sidorov, R. A., Zhukov, A. V., Pchelkin, V. P., Vereshchagin, A. G., Tsydendambaev, V. D. (2014). Content and Fatty Acid Composition of Neutral Acylglycerols in Euonymus Fruits. Journal of the American Oil Chemists’ Society, 91 (5), 805–814. doi: 10.1007/s11746-014-2425-2
Hernández-Ortega, M., Ortiz-Moreno, A., Hernández-Navarro, M. D., Chamorro-Cevallos, G., Dorantes-Alvarez, L., Necoechea-Mondragón, H. (2012). Antioxidant, Antinociceptive, and Anti-Inflammatory Effects of Carotenoids Extracted from Dried Pepper (Capsicum annuumL.). Journal of Biomedicine and Biotechnology, 2012, 1–10. doi: 10.1155/2012/524019
Kumar, R., Kumar, G. P., Chaurasia, O., Bala Singh, S. (2011). Phytochemical and Pharmacological Profile of Seabuckthorn Oil: A Review. Research Journal of Medicinal Plant, 5 (5), 491–499. doi: 10.3923/rjmp.2011.491.499
Mazurec, S. I., Kovalyov, S. V., Rudnyk, A. M. (2012). Phytochemical study of the lipophilic fraction from the leaves of ordinary hops. Zaporizhzhia Medical Journal, 72 (3), 96–99.
Marchyshyn, S., Krasulya, N., Kulitska, M., Ostrovska, G. (2011). Investigation of the lipophilic fraction of the herb of the chammeria narrow-gauge. Farmacevtuchnyj chasopys, 1, 18–21.
Sidorov, R. A. (2013). Composition of greasy oils of berry bushes fruits. Moscow, 22.
Pharmaceutical Encyclopedia (2010). Kyiv: MORION, 1632
Poddubny, N. P., Sampiyev, A. M. (2000). Beta-carotene: experience and perspectives in medicine. Krasnodar, 34.
Yakovleva, L. V., Tkachova, O. V., Gladuk, E. V. (2003). Study of the therapeutic effect of the almond ointment on the model of contact turpentine dermatitis in rats. Collection of scientific works of NMAPO named after P. L. Shupyk, 12 (1), 1000–1005.
Happe, M. R., Battafarano, D. F., Dooley, D. P., Rennie, T. A., Murphy, F. T., Casey, T. J., Ward, J. A. (2002). Validation of the Diesse Mini-Ves Erythrocyte Sedimentation Rate (ESR) Analyzer Using the Westergren ESR Method in Patients With Systemic Inflammatory Conditions. American Journal of Clinical Pathology, 118 (1), 14–17. doi: 10.1309/yhyl-8xth-5jm2-43ej
Kamyshnikov, B. C. (2009). Reference book for clinical and biochemical research and laboratory diagnostics. MED press-inform, 920.
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