ORIGINAL EFFECTIVE, SAFE TECHNIQUE OF OBTAINING PLATELET RICH PLASMA BY CENTRIFUGATION OF THE BLOOD PLASMA IN MODIFIED SYRINGE CONTAINERS

Sergiy Chetverikov, Dmitro Atanasov

Abstract


The aim: to develop, substantiate an effective and safe technology for producing PRP (platelet rich plasma). To quantify the substrate based on the recommended centrifugation protocols.

Materials and methods: the effectiveness of the original harvesting protocol was evaluated by quantifying the number of platelets. The proposed technique is formed basing on the basic principles of double centrifugation of whole blood in test tubes with anticoagulant, separation with the release of a plasma layer with a high content of platelets.

The centrifuging mode for quantifying the effectiveness of the substrate was selected according to recommendations based on a study confirming maximum efficiency (160g×10min + 250g×15min).

For quantitative evaluation, blood was collected from 10 healthy volunteers (7 men, 3 women) with an average age of 26.0±2.6, and centrifuged in standard mode. Quantitative evaluation of platelets of whole blood and the obtained PRP substrate was carried out with a semi-automatic analyzer.

Results: the proposed technique is based on the use as a container for centrifuging a syringe with a LuerLock design, which is hermetically sealed with a congruent plug, adapted by the external size of the centrifuge rotor bowl. Phase selection after centrifugation was performed by aspiration of the syringe contents after centrifugation is performed through a three-way valve. The substrate was obtained by repeated centrifugation of the contents, which allows obtaining a variable volume and platelet concentration in PRP. The amount of platelets (PLT) of whole blood is 227.0±57.0 thousand per ml. PLT PRP 945.0±279.0 thousand per ml.

Conclusions: the proposed method of separation of whole blood with the release of the platelet rich plasma demonstrates high efficiency, which corresponds to the level of increasing the number of platelets in reducing the volume at the level of the best ready-made solutions.

The equipment is economical and does not require highly specialized equipment and consumables. The proposed technique provides a wide choice to the performer in the received volume of the substrate.


Keywords


platelet rich plasma; platelets; red blood cells; centrifugation; syringe; Discofix; combi-stopper; harvesting

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References


Dhurat, R., Sukesh, M. (2014). Principles and methods of preparation of platelet-rich plasma: A review and author′s perspective. Journal of Cutaneous and Aesthetic Surgery, 7 (4), 189. doi: https://doi.org/10.4103/0974-2077.150734

Martinez-Zapata, M. J., Martí-Carvajal, A. J., Solà, I., Expósito, J. A., Bolíbar, I., Rodríguez, L. et. al. (2016). Autologous platelet-rich plasma for treating chronic wounds. Cochrane Database of Systematic Reviews. doi: https://doi.org/10.1002/14651858.cd006899.pub3

Sister, D. (2016). PRP: the new frontier in regenerative medicine and aesthetic medicine. Firence, 158.

Fareed, W. M., Tandon, P., Ahmad Z, E., Gazal, G., Hussain Khan, A., Zaidi, T. et. al. (2017). Efficacy of Blood and Its Products-boon for Oral Surgeons: Review. Journal of Universal Surgery, 05 (01). doi: https://doi.org/10.21767/2254-6758.100071

Joshi Jubert, N., Rodríguez, L., Reverté-Vinaixa, M. M., Navarro, A. (2017). Platelet-Rich Plasma Injections for Advanced Knee Osteoarthritis: A Prospective, Randomized, Double-Blinded Clinical Trial. Orthopaedic Journal of Sports Medicine, 5 (2), 232596711668938. doi: https://doi.org/10.1177/2325967116689386

Parizzi, N. G., Rubini, O. Á., Almeida, S. H. M. de, Ireno, L. C., Tashiro, R. M., Carvalho, V. H. T. de. (2017). Effect of platelet-rich plasma on polypropylene meshes implanted in the rabbit vagina: histological analysis. International Braz j Urol, 43 (4), 746–752. doi: https://doi.org/10.1590/s1677-5538.ibju.2016.0177

Dönmez, M. İ., İnci, K., Zeybek, N. D., Doğan, H. S., Ergen, A. (2016). The Early Histological Effects of Intravesical Instillation of Platelet-Rich Plasma in Cystitis Models. International Neurourology Journal, 20 (3), 188–196. doi: https://doi.org/10.5213/inj.1632548.274

McCarrel, T. M., Minas, T., Fortier, L. A. (2012). Optimization of Leukocyte Concentration in Platelet-Rich Plasma for the Treatment of Tendinopathy. The Journal of Bone & Joint Surgery, 94 (19), e143. doi: https://doi.org/10.2106/jbjs.l.00019

Bausset, O., Giraudo, L., Veran, J., Magalon, J., Coudreuse, J.-M., Magalon, G. et. al. (2012). Formulation and Storage of Platelet-Rich Plasma Homemade Product. BioResearch Open Access, 1 (3), 115–123. doi: https://doi.org/10.1089/biores.2012.0225

Dhurat, R., Sukesh, M. (2014). Principles and methods of preparation of platelet-rich plasma: A review and author′s perspective. Journal of Cutaneous and Aesthetic Surgery, 7 (4), 189. doi: https://doi.org/10.4103/0974-2077.150734

Fitzpatrick, J., Bulsara, M. K., McCrory, P. R., Richardson, M. D., Zheng, M. H. (2017). Analysis of Platelet-Rich Plasma Extraction. Orthopaedic Journal of Sports Medicine, 5 (1), 232596711667527. doi: https://doi.org/10.1177/2325967116675272

Kushida, S., Kakudo, N., Morimoto, N., Hara, T., Ogawa, T., Mitsui, T., Kusumoto, K. (2014). Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems. Journal of Artificial Organs, 17 (2), 186–192. doi: https://doi.org/10.1007/s10047-014-0761-5

Kececi, Y., Ozsu, S., Bilgir, O. (2014). A cost-effective method for obtaining standard platelet-rich plasma. Wounds, 26, 232–238.

Foster, T. E., Puskas, B. L., Mandelbaum, B. R., Gerhardt, M. B., Rodeo, S. A. (2009). Platelet-Rich Plasma. The American Journal of Sports Medicine, 37 (11), 2259–2272. doi: https://doi.org/10.1177/0363546509349921

Perez, A. G. M., Lana, J. F. S. D., Rodrigues, A. A., Luzo, A. C. M., Belangero, W. D., Santana, M. H. A. (2014). Relevant Aspects of Centrifugation Step in the Preparation of Platelet-Rich Plasma. ISRN Hematology, 2014, 1–8. doi: https://doi.org/10.1155/2014/176060

Degen, R. M., Bernard, J. A., Oliver, K. S., Dines, J. S. (2016). Commercial Separation Systems Designed for Preparation of Platelet-Rich Plasma Yield Differences in Cellular Composition. HSS Journal ®, 13 (1), 75–80. doi: https://doi.org/10.1007/s11420-016-9519-3

Magalon, J., Bausset, O., Serratrice, N., Giraudo, L., Aboudou, H., Veran, J. et. al. (2014). Characterization and Comparison of 5 Platelet-Rich Plasma Preparations in a Single-Donor Model. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 30 (5), 629–638. doi: https://doi.org/10.1016/j.arthro.2014.02.020

Yin, W., Xu, H., Sheng, J., Zhu, Z., Jin, D., Hsu, P. et. al. (2017). Optimization of pure platelet-rich plasma preparation: A comparative study of pure platelet-rich plasma obtained using different centrifugal conditions in a single-donor model. Experimental and Therapeutic Medicine, 14 (3), 2060–2070. doi: https://doi.org/10.3892/etm.2017.4726

Cavallo, C., Roffi, A., Grigolo, B., Mariani, E., Pratelli, L., Merli, G. et. al. (2016). Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules. BioMed Research International, 2016, 1–7. doi: https://doi.org/10.1155/2016/6591717




DOI: http://dx.doi.org/10.21303/2504-5679.2019.00844

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ISSN 2504-5679 (Online), ISSN 2504-5660 (Print)