MICROBIOLOGICAL STUDY OF A PERSPECTIVE HEPATOPROTECTIVE AGENT BASED ON DRY EXTRACT FROM PRUNUS DOMESTICA FRUITS

Nataliia Filimonova, Bashar Jabbar Ali Sahlanee, Igor Senyuk, Alevtyna Kononenko

Abstract


One of the main directions of improving the concept of treatment of diseases of the gastrointestinal tract and liver, which is accompanied by dysfunction of the hepatobiliary system and intestines, was the search and development of herbal remedies with hepatoprotective effect with a laxative effect and concomitant prebiotic activity.

Aim of the research: to study the antimicrobial and prebiotic properties of the studied phytosubstance obtained from the fruits of Prunus domestica.

Materials and methods. The study of the antimicrobial activity of the test sample of plum fruit extract with fibers was performed in vitro by the method of multiple serial dilutions. The reference strains of the following microorganisms were used as the microbiological model: S.aureus-ATCC-25923, E.сoli-ATCC-25922, P. aéruginosa-ATCC-27853, B. subtilis-ATCC-6633, C.albicans-ATCC-885653.

The determination of the prebiotic properties of the studied sample was carried out by the method of cultivation of bacteria Bifidobacterium bifidum No. 1 and L. rhamnosus R0011 ND on nutrient media, which included the composition of plum fruit with fibers.

Results. Studies on the antimicrobial activity of plum fruit extract with fibers showed that the studied phytosubstance does not reveal antagonistic properties both with respect to gram-positive and gram-negative bacteria, as well as in the case of Candida fungi. The results of studies on the study of the prebiotic properties of the studied extract confirmed the improvement of the main physiological parameters of the culture of bifidobacteria strain Bifidobacterium bifidum No. 1 and the strain lactobacillus L. rhamnosus R0011 ND when the fruit plums with fibers were added to the culture medium.

Conclusions. The conducted studies allowed to establish the prebiotic properties of the studied extract of plum fruits with fibers, which proves the promise of its use in complex therapy schemes for gastrointestinal pathology.


Keywords


hepatoprotective agent; fruits of Prunus domestica; bifidobacteria; lactobacilli; prebiotic properties; antimicrobial activity

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DOI: http://dx.doi.org/10.21303/2504-5679.2019.00870

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