Nataliia Filimonova, Bashar Jabbar Ali Sahlanee, Igor Senyuk, Alevtyna Kononenko


One of the main directions of improving the concept of treatment of diseases of the gastrointestinal tract and liver, which is accompanied by dysfunction of the hepatobiliary system and intestines, was the search and development of herbal remedies with hepatoprotective effect with a laxative effect and concomitant prebiotic activity.

Aim of the research: to study the antimicrobial and prebiotic properties of the studied phytosubstance obtained from the fruits of Prunus domestica.

Materials and methods. The study of the antimicrobial activity of the test sample of plum fruit extract with fibers was performed in vitro by the method of multiple serial dilutions. The reference strains of the following microorganisms were used as the microbiological model: S.aureus-ATCC-25923, E.сoli-ATCC-25922, P. aéruginosa-ATCC-27853, B. subtilis-ATCC-6633, C.albicans-ATCC-885653.

The determination of the prebiotic properties of the studied sample was carried out by the method of cultivation of bacteria Bifidobacterium bifidum No. 1 and L. rhamnosus R0011 ND on nutrient media, which included the composition of plum fruit with fibers.

Results. Studies on the antimicrobial activity of plum fruit extract with fibers showed that the studied phytosubstance does not reveal antagonistic properties both with respect to gram-positive and gram-negative bacteria, as well as in the case of Candida fungi. The results of studies on the study of the prebiotic properties of the studied extract confirmed the improvement of the main physiological parameters of the culture of bifidobacteria strain Bifidobacterium bifidum No. 1 and the strain lactobacillus L. rhamnosus R0011 ND when the fruit plums with fibers were added to the culture medium.

Conclusions. The conducted studies allowed to establish the prebiotic properties of the studied extract of plum fruits with fibers, which proves the promise of its use in complex therapy schemes for gastrointestinal pathology.


hepatoprotective agent; fruits of Prunus domestica; bifidobacteria; lactobacilli; prebiotic properties; antimicrobial activity

Full Text:



Ubeeva, E. A., Nickolaev, S. M., Ubeeva, I. P. (2017). The main directions of phytotherapy of liver diseases. Vestnik Medicine and Pharmacy, 3, 3–9.

Ubeeva, I. P., Nickolaev, S. M., Botoeva, Е. А. (2009). Herbal medicine for diseases of the hepatobiliary system. Irkutsk: RIO IGIUAs, 109.

Grodinsky, A. M. (Ed.) (1992). Medicinal plants: Encyclopedic reference book. Kyiv: "Ukrainian Encyclopedia" Publishing House named after M. P. Bazhan, Ukr. Production and Commercial Center "Olympus", 544.

Chernykh, V. P. (Ed.) (2010). Pharmaceutical encyclopedia. Kyiv: MORION, 1952.

The State Pharmacopoeia of Ukraine (2008). State Enterprise “Ukrainian scientific Pharmacopoeia center”. Kharkiv: RІRЕG, 620 p.

Basim, M. S., Lenchik, L. V., Caidov, N. B. (2017). Prunus Domestica fruits – perspective row for drugs development. Nauka i innovaciya. Seriya estestvennyh nauk, 4, 42–48.

Upyr, T., Basim Mohammed, S., Bashar, A.-J. A. S., Lenchyk, L., Senyuk, I., Kyslychenko, V. (2018). Phytochemical and pharmacological study of polysaccharide complexes of prunus domestica fruit. ScienceRise: Pharmaceutical Science, 3 (13), 32–37. doi: http://doi.org/10.15587/2519-4852.2018.135825

Celik, F., Gundogdu, M., Alp, S., Muradoglu, F., Ercişli, S., Gecer, M. K., Canan, I. (2017). Determination of phenolic compounds, antioxidant capacity and organic acids contents of Prunus domestica L., Prunus cerasifera Ehrh. and Prunus spinosa L. fruits by HPLC. Acta Chromatographica, 29 (4), 507–510. doi: http://doi.org/10.1556/1326.2017.00327

Kayano, S., Kikuzaki, H., Fukutsuka, N., Mitani, T., Nakatani, N. (2002). Antioxidant Activity of Prune (Prunus domesticaL.) Constituents and a New Synergist. Journal of Agricultural and Food Chemistry, 50 (13), 3708–3712. doi: http://doi.org/10.1021/jf0200164

Senyuk, I. V., Bashar, A.-S. J., Lenchyk, L. V. (2017). Investigation of different substances catharic properties made from Prunus domestica. Ukrainian biopharmaceutical journal, 5 (52), 21–25. doi: http://doi.org/10.24959/ubphj.17.134

Senjuk, I. V., Al Sahlani, B. J., Basim, M. S. (2018) Study of hepatoprotective effect of extracts from Prunus domestica fruits. Pharmaceutical review, 4 (48), 57–61. doi: http://doi.org/10.11603/2312-0967.2018.4.9692

Tochilina, O. A., Chastoedova, I. A. (2013). Intestinal dysbiosis and enzyme-excretory function in young children after use antibiotics and methods of correction. Human ecology, 9, 44–48.

Gibson, G. R., Probert, H. M., Loo, J. V., Rastall, R. A., Roberfroid, M. B. (2004). Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutrition Research Reviews, 17 (2), 259–275. doi: http://doi.org/10.1079/nrr200479

Astashkina, A. P. (2010). Modern views on the biological role of bifidobacteria and lactobacilli. Vestnik VGU. Series: Chemistry. Biology, Pharmacy, 1, 133–139.

Anderson, R. C., Cookson, A. L., McNabb, W. C., Park, Z., McCann, M. J., Kelly, W. J., Roy, N. C. (2010). Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation. BMC Microbiology, 10 (1), 316. doi: http://doi.org/10.1186/1471-2180-10-316

Lifshitz, L., Zakharova, I. N., Dmitrieva, Y. A. (2017). Effect of intestinal microbiome in norm and pathology on human health. Medical Council, 1, 155–159. doi: http://doi.org/10.21518/2079-701x-2017-1-155-159

Volyansky, Yu. L., Gritsenko, I. S., Shirobokov, V. P. et. al. (2004). Study of specific activity of antimicrobial drugs. Kyiv, 40.

Cheremushkina, I. V., Glushchenko, A. S., Anohinа, E. P., Chigirina, N. A., Cherenkov D. A., Slepokurov, A. A. et. al. (2010). Biotechnology mannozosoderzhaschih hydrolysates and study of prebiotic properties of mannose. Biotechnology, 5, 56–61.

Holt, J., Krieg, N., Smith, P., Staley, G., Williams, C. (1997). The determinant of Berdji bacteria. Vol. 1. Moscow: Mir, 432.

Holt, J., Krieg, N., Smith, P., Staley, G., Williams, C. (1997). The determinant of Berdji bacteria. Vol. 2. Moscow: Mir, 359.

Delgado-Adámez, J., Fernández-León, M. F., Velardo-Micharet, B., González-Gómez, D. (2012). In vitro assays of the antibacterial and antioxidant activity of aqueous leaf extracts from different Prunus salicina Lindl. cultivars. Food and Chemical Toxicology, 50 (7), 2481–2486. doi: http://doi.org/10.1016/j.fct.2012.02.024

Sójka, M., Kołodziejczyk, K., Milala, J., Abadias, M., Viñas, I., Guyot, S., Baron, A. (2015). Composition and properties of the polyphenolic extracts obtained from industrial plum pomaces. Journal of Functional Foods, 12, 168–178. doi: http://doi.org/10.1016/j.jff.2014.11.015

Kravchenko, V. M., Sahlani, B. J. A., Senyuk, I. V. (2018). Investigation of acute toxicity of plum home fruit extracts. Prospects for the development of medicine in EU countries and Ukraine. Wloclawek, 109–112.

DOI: http://dx.doi.org/10.21303/2504-5679.2019.00870


  • There are currently no refbacks.

Copyright (c) 2019 Nataliia Filimonova, Bashar Jabbar Ali Sahlanee, Igor Senyuk, Alevtyna Kononenko

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

ISSN 2504-5679 (Online), ISSN 2504-5660 (Print)