PECULIARITIES OF TLR-2 (ARG753GLN) AND TLR-4 (ASP299GLY) POLYMORPHISM PREVALENCE IN PATIENTS WITH ACUTE BRUCELLEIS AND CARDIOVASCULAR SYSTEM DISEASES

Elchin Mammad oglu Huseynov

Abstract


Currently, there are single data on the relationship between TLR-2 polymorphisms (Arg753Gln) and TLR-4 (Asp299Gly) polymorphisms and susceptibility to brucellosis. Therefore, the aim of the study was to determine the frequency of TLR-2 (Arg753Gln) and TLR-4 (Asp299Gly) polymorphisms in patients with acute brucellosis with cardiovascular lesions in the Republic of Azerbaijan.

Materials and methods: 178 patients with a brucellosis clinic were examined. According to the criteria for inclusion in the study, only 120 people fully met all the criteria, which made up the main group. The control group consisted of 30 healthy individuals. TLR-2 (Arg753Gln) and TLR-4 (Asp299Gly) polymorphisms were also determined for all patients in both groups. In order to assess the state of the cardiovascular system, an electrocardiogram was recorded, blood pressure was measured, and an ultrasound scan of the heart was performed for all patients.

Results: it was found that 93 patients (77.50±3.13 %) with acute brucellosis had some or other signs of impairment in the work of the cardiovascular system, identified clinically or as a result of functional examination. Among carriers of the Asp / Gly genotype of the TLR-4 gene, an increased risk of brucellosis with CVS diseases was determined (χ2=30.19; p <0.0001; OR=24.29; 95 % CI [5.45 – 108.37]), while the carriage of the homozygous genotype Asp / Asp, by contrast, had a protective effect on the development of brucellosis (OR=0.06, 95 % CI [0.02 – 0.20]). Among the carriers of the Arg / Gln genotype and the Gln / Gln genotype of the TLR-2 gene, a significantly increased risk of brucellosis with CVS diseases was determined (χ2=5.68; p=0.02; OR=3.10; 95 % CI [0.99 – 9.67]) and (OR=2.48; 95 % CI [0.53 – 11.61]), respectively. While the carriage of the homozygous Arg / Arg genotype, by contrast, was rarely seen in patients with brucellosis (OR=0.28, 95 % CI [0.10 – 0.74]).

Conclusions: The Asp / Gly genotype of the TLR-4 gene was 12.7 times more frequently detected in patients with acute brucellosis with CVS diseases than in healthy individuals and 9.5 times more often than in patients without CVS diseases (p<0.05). The Arg / Gln genotype of the TLR-2 gene was 2.4 times more frequently detected in patients with acute brucellosis with CVS diseases than in healthy individuals (p<0.05).


Keywords


acute brucellosis; TLR-4; TLR-2; polymorphism; cardiovascular system

Full Text:

PDF

References


Najafi, N., Davoudi, A., Hassantabar, S. R., Haddadi, R. (2018). Investigating the Epidemiologic, Laboratory, and Clinical Features of Brucellosis Patients Hospitalized in the North of Iran During 2009–2014. Archives of Clinical Infectious Diseases, 13 (2). doi: http://doi.org/10.5812/archcid.61012

Mugahi, S., Nashibi, R., Alavi, S. M., Gharkholu, S., Najafi, K. (2014). Epidemiological Features, Clinical Manifestation and Laboratory Findings of Patients With Brucellosis. Archives of Clinical Infectious Diseases, 9 (1). doi: http://doi.org/10.5812/archcid.17270

Nourbakhsh, F., Borooni, S., Barangi, S., Tajbakhsh, E. (2017). Diagnosis of clinical and laboratory findings of brucellosis in Isfahan. International Archives of Health Sciences, 4 (2), 48. doi: http://doi.org/10.4103/iahs.iahs_1_17

Garcell, H. G., Garcia, E. G., Pueyo, P. V., Martín, I. R., Arias, A. V., Alfonso Serrano, R. N. (2016). Outbreaks of brucellosis related to the consumption of unpasteurized camel milk. Journal of Infection and Public Health, 9 (4), 523–527. doi: http://doi.org/10.1016/j.jiph.2015.12.006

Kazak, E., Akalın, H., Yılmaz, E., Heper, Y., Mıstık, R., Sınırtaş, M. et. al. (2016). Brucellosis: a retrospective evaluation of 164 cases. Singapore Medical Journal, 57 (11), 624–629. doi: http://doi.org/10.11622/smedj.2015163

Bukharie, H. A. (2009). Clinical features, complications and treatment outcome of Brucella infection: Ten years' experience in an endemic area. Tropical Journal of Pharmaceutical Research, 8 (4). doi: http://doi.org/10.4314/tjpr.v8i4.45221

Nassaji, M., Govhary, A., Ghorbani, R. (2015). Epidemiological, clinical and laboratory findings in adult patients with acute brucellosis: A case-control study. Acta Medica Mediterranea, 1319–1325. Available at: http://eprints.semums.ac.ir/id/eprint/78

Sofian, M., Aghakhani, A., Velayati, A. A., Banifazl, M., Eslamifar, A., Ramezani, A. (2008). Risk factors for human brucellosis in Iran: a case–control study. International Journal of Infectious Diseases, 12 (2), 157–161. doi: http://doi.org/10.1016/j.ijid.2007.04.019

Lagadinou, M., Mplani, V., Velissaris, D., Davlouros, P., Marangos, M. (2019). Myocarditis Caused by Brucella melitensis in the Absence of Endocarditis: Case Report and Review of the Literature. Case Reports in Medicine, 2019, 1–4. doi: http://doi.org/10.1155/2019/3701016

Pandit, V. R., Seshadri, S., Valsalan, R., Bahuleyan, S., Vandana, K. E., Kori, P. (2010). Acute brucellosis complicated by fatal myocarditis. International Journal of Infectious Diseases, 14 (4), e358–e360. doi: http://doi.org/10.1016/j.ijid.2009.05.013

Yurko, K. V. (2015). Prevalence of TLR-4 gene polymorphism in patients co-infected with HIV/HCV. ScienceRise, 11 (3 (16)), 86–89. doi: http://doi.org/10.15587/2313-8416.2015.54495

Cai, Y., Peng, Y.-H., Tang, Z., Guo, X.-L., Qing, Y.-F., Liang, S.-H. et. al. (2014). Association of Toll-like receptor 2 polymorphisms with gout. Biomedical Reports, 2 (2), 292–296. doi: http://doi.org/10.3892/br.2014.224

Kawai, T., Akira, S. (2010). The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology, 11 (5), 373–384. doi: http://doi.org/10.1038/ni.1863

Tongtawee, T., Bartpho, T., Kaewpitoon, S., Kaewpitoon, N., Dechsukhum, C., Leeanansaksiri, W. et. al. (2018). Genetic polymorphisms in TLR1, TLR2, TLR4, and TLR10 of Helicobacter pylori-associated gastritis. European Journal of Cancer Prevention, 27 (2), 118–123. doi: http://doi.org/10.1097/cej.0000000000000347

Ferrero, M. C., Hielpos, M. S., Carvalho, N. B., Barrionuevo, P., Corsetti, P. P., Giambartolomei, G. H. et. al. (2013). Key Role of Toll-Like Receptor 2 in the Inflammatory Response and Major Histocompatibility Complex Class II Downregulation in Brucella abortus-Infected Alveolar Macrophages. Infection and Immunity, 82 (2), 626–639. doi: http://doi.org/10.1128/iai.01237-13

Oliveira, S. C., de Oliveira, F. S., Macedo, G. C., de Almeida, L. A., Carvalho, N. B. (2008). The role of innate immune receptors in the control of Brucella abortus infection: Toll-like receptors and beyond. Microbes and Infection, 10 (9), 1005–1009. doi: http://doi.org/10.1016/j.micinf.2008.07.005

Rezazadeh, M., Hajilooi, M., Rafiei, A., Haidari, M., Nikoopour, E., Kerammat, F. et. al. (2006). TLR4 polymorphism in Iranian patients with brucellosis. Journal of Infection, 53 (3), 206–210. doi: http://doi.org/10.1016/j.jinf.2005.10.018

Nguyen, M.-T., Peisl, L., Barletta, F., Luqman, A., Götz, F. (2018). Toll-Like Receptor 2 and Lipoprotein-Like Lipoproteins Enhance Staphylococcus aureus Invasion in Epithelial Cells. Infection and Immunity, 86 (8). doi: http://doi.org/10.1128/iai.00627-18

Xue, X., Qiu, Y., Jiang, D., Jin, T., Yan, M., Zhu, X., Chu, Y. (2017). The association analysis of TLR2 and TLR4 gene with tuberculosis in the Tibetan Chinese population. Oncotarget, 8 (68). doi: http://doi.org/10.18632/oncotarget.22996

Zhao, J., Shang, H., Cao, X., Huang, Y., Fang, X., Zhang, S. et. al. (2017). Association of polymorphisms in TLR2 and TLR4 with asthma risk. Medicine, 96 (35), e7909. doi: http://doi.org/10.1097/md.0000000000007909




DOI: http://dx.doi.org/10.21303/2504-5679.2019.00923

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Elchin Mammad oglu Huseynov

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

ISSN 2504-5679 (Online), ISSN 2504-5660 (Print)