ASSESSMENT OF RICE SORTS AND HYBRIDS ON THE SALT TOLERANCE USING LABORATORY METHODS

  • Pavel Kostylev FSBSI All-Russian Research Institute of Grain Crops of I. G. Kalinenko, Russian Federation
  • Ekaterina Kudashkina Azov-Blacksea Engineering Institute VSBEI DSAU, Russian Federation
Keywords: rice, sort, sample, salt tolerance, DNA markers, PCR, Saltol

Abstract

The article presents the results of the analysis of salt tolerance in rice samples by the laboratory methods at a concentration of NaCl – 1,5 %. The plants were grown in an incubator at temperature 26 °C. After 15 days, we measured the length of roots and shoots; the germination of seeds was counted. For the analysis of hybrids sensitive to salt Krasnodar varieties Novator with lines IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, NSIC Rc 106 – donor locus salt tolerance Saltol, using markers RM 493 and RM 7075. 90 plants were selected from the F2 plants which are analyzed by PCR, and the results of DNA analysis identified 6 of them as salt tolerant samples homozygous for the desired locus. From their offspring was selected the promising form to create salt-tolerant rice sorts.

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Author Biographies

Pavel Kostylev, FSBSI All-Russian Research Institute of Grain Crops of I. G. Kalinenko

Laboratory of breeding, seed production and cultivation technology of rice

Ekaterina Kudashkina, Azov-Blacksea Engineering Institute VSBEI DSAU

Department of Agronomy and Сrop Breeding

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Published
2016-06-13
How to Cite
Kostylev, P., & Kudashkina, E. (2016). ASSESSMENT OF RICE SORTS AND HYBRIDS ON THE SALT TOLERANCE USING LABORATORY METHODS. EUREKA: Life Sciences, (2), 37-43. https://doi.org/10.21303/2504-5695.2016.00106
Section
Agricultural and Biological Sciences