ASSESSMENT OF RICE SORTS AND HYBRIDS ON THE SALT TOLERANCE USING LABORATORY METHODS

Pavel Kostylev, Ekaterina Kudashkina

Abstract


The article presents the results of the analysis of salt tolerance in rice samples by the laboratory methods at a concentration of NaCl – 1,5 %. The plants were grown in an incubator at temperature 26 °C. After 15 days, we measured the length of roots and shoots; the germination of seeds was counted. For the analysis of hybrids sensitive to salt Krasnodar varieties Novator with lines IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, NSIC Rc 106 – donor locus salt tolerance Saltol, using markers RM 493 and RM 7075. 90 plants were selected from the F2 plants which are analyzed by PCR, and the results of DNA analysis identified 6 of them as salt tolerant samples homozygous for the desired locus. From their offspring was selected the promising form to create salt-tolerant rice sorts.


Keywords


rice; sort; sample; salt tolerance; DNA markers; PCR; Saltol

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References


Negrão, S., Courtois, B., Ahmadi, N., Abreu, I., Saibo, N., Oliveira, M. M. (2011). Recent Updates on Salinity Stress in Rice: From Physiological to Molecular Responses. Critical Reviews in Plant Sciences, 30 (4), 329–377. doi: 10.1080/07352689.2011.587725

Ladatko, N. A. (2006). Morpho-physiological features varieties rice, conditional on their resistance to soil salinity, in connection with the development of methods for evaluating the salt tolerance breeding samples. Krasnodar, 190.

Singh, D. (2011). Marker assisted selection and crop management for salt tolerance: A review. African journal of biotechnology, 10 (66). doi: 10.5897/ajb11.049

Islam, M. R., Salam, M. A., Hassan, L. et. al (2011). QTL mapping for salinity tolerance at seedling stage in rice. J. Agric. Food Technol., 23, 137–146.

Ren, Z.-H., Gao, J.-P., Li, L.-G., Cai, X.-L., Huang, W., Chao, D.-Y. et. al (2005). A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nature Genetics, 37 (10), 1141–1146. doi: 10.1038/ng1643

Sabouri, H., Rezai, A. M., Moumeni, A., Kavousi, A., Katouzi, M., Sabouri, A. (2009). QTLs mapping of physiological traits related to salt tolerance in young rice seedlings. Biologia Plantarum, 53 (4), 657–662. doi: 10.1007/s10535-009-0119-7

Takehisa, H., Shimodate, T., Fukuta, Y., Ueda, T., Yano, M., Yamaya, T. et. al (2004). Identification of quantitative trait loci for plant growth of rice in paddy field flooded with salt water. Field Crops Research, 89 (1), 85–95. doi: 10.1016/j.fcr.2004.01.026

Platten, J. D., Egdane, J. A., Ismail, A. M. (2013). Salinity tolerance, Na+ exclusion and allele mining of HKT1;5 in Oryza sativa and O. glaberrima: many sources, many genes, one mechanism? BMC Plant Biology, 13 (1), 32. doi: 10.1186/1471-2229-13-32

Kostylev, P. I. (2011). Methods of breeding, seed production and varietal rice farming techniques. Rostov-on-Don: JSC “Book”, 288.

Thomson, M. J., Ocampo, M., Egdane, J., Rahman, M. A., Sajise, A. G., Adorada, D. L. et. al (2010). Characterizing the Saltol Quantitative Trait Locus for Salinity Tolerance in Rice. Rice, 3 (2-3), 148–160. doi: 10.1007/s12284-010-9053-8

Kudashkina, E. B., Kostylev, P. I. (2015). The study of salt tolerance of rice laboratory methods. The role of botanic gardens in the conservation and monitoring of biodiversity. Rostov-on-Don: Publishing House of the SFU, 401–404.

Kudashkina, E. B., Kostylev, P. I. (2015). Screening of rice accessions to salt tolerance. Contribution Vavilov society of geneticists and breeders in the innovative development of the Russian Federation. Cuban SAU, 70–72.

Usatov, A. V., Alabushev, A. V., Kostylev, P. I., Azarin, K. V., Makarenko, M. S., Usatova, O. A. (2015). Introgression the SalTol QTL into the Elite Rice Variety of Russia by Marker-Assisted Selection. American Journal of Agricultural and Biological Sciences, 10 (4), 165–169. doi: 10.3844/ajabssp.2015.165.169

Azarin, K. V., Alabushev, A. V., Usatov, A. V., Kostylev, P. I., Kolokolova, N. S., Usatova, O. A. (2016). Effects of Salt Stress on Ion Balance at Vegetative Stage in Rice (Oryza sativa L.). OnLine Journal of Biological Sciences, 16 (1), 76–81. doi: 10.3844/ojbsci.2016.76.81

Jeung, J. U., Hwang, H. G., Moon, H. P., Jena, K. K. (2005). Fingerprinting temperate japonica and tropical indica rice genotypes by comparative analysis of DNA markers. Euphytica, 146 (3), 239–251. doi: 10.1007/s10681-005-9022-2

Lang, N. T., Buu, B. C., Ismail, A. M. (2008). Molecular mapping and marker assisted selection for salt tolerance in rice (Oryza sativa L.). Omonrice, 16, 50–56.

Elahi, C. M. F., Seraj, Z. I., Rasul, N. M. et. al; Islam, A. S. (Ed.) (2004). “Breeding rice for salinity tolerance using the Pokkali allele: finding a linked marker,” in In Vitro Culture, Transformation and Molecular Markers for Crop Improvement. Science Publisher, NH, USA, 157–169.

Mackill, D. J. (2007). Molecular markers and marker-assisted selection in rice. Vol. 2: Genomics Applications in Crops. Springer, New York, NY, USA, 147–168. doi: 10.1007/978-1-4020-6297-1_7

Linh, L. H., Linh, T. H., Xuan, T. D., Ham, L. H., Ismail, A. M., Khanh, T. D. (2012). Molecular Breeding to Improve Salt Tolerance of Rice (Oryza sativaL.) in the Red River Delta of Vietnam. International Journal of Plant Genomics, 2012, 1–9. doi: 10.1155/2012/949038

Kostylev, P. I., Red’kin, A. A., Krasnova, E. V., Usatov, A. V., Makarenko, M. S. (2015). Effect of salt tolerance genes on quantitative characters of rice under the Rostov region. Actual problems of biology, nanotechnology and medicine. Rostov-on-Don, 92–94.




DOI: http://dx.doi.org/10.21303/2504-5695.2016.00106

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