CYTOGENETIC EVALUATION OF THE DRINKING WATER TOXICITY
Abstract
There was considered the use of biotesting for the assessment of the quality of drinking water from the different water supply sources (artesian, packaged and water-pipe one). The method consists in determination of the toxicants action on the specially selected organisms in the standard conditions with registration of changes at behavior, physiological, cellular and subcellular level using hematological indices of fish, frogs, rats and the lymphocyte cultures of the peripheral human blood. Physical-chemical methods determine only the presence and number of chemical elements in the tested water samples because of the very large number of possible combinations of chemical compounds in water solutions (more than 75 million combinations), including behavior of anthropogenic compounds and the natural vulnerability of the water ecosystems to the combined effects from its toxic influence.
As the optimal set of determination of the some structural and functional changes of cell genome as the result of the toxic influence of combination of the chemical compounds in the water solutions was offered the micronuclear test and leukocytic formula of the fish, frog and rat blood as biomarker. The reaction of fish, frog, rat test-organisms on the toxic irritation is presented in the change of qualitative content of the cells of peripheral blood. There were demonstrated the prospects of the use of hematological indices of the following test-organisms: Danio rerio fishes, Xenopus clawed frogs, Wistar rats and also the lymphocytes cultures of the human peripheral blood. The special attention was paid to the assessment of the risk for human health of the toxic substances in drinking water, genotoxicity and cytotoxicity that are revealed using hematological indices of animal cells. The universality of cellular organization opens the wide possibilities for toxicological studies using peripheral blood of the different groups of animals (fish, frogs, rats), human lymphocyte cultures and allow assume the following possibility of extrapolation of the received results on human organism
Downloads
References
Arkhipchuk, V. V., Goncharuk, V. V. (2004). Problemy kachestva pit'evyh butylirovannyh vod. Himija i tehnologija vody, 26 (4), 403–414.
Goncharuk, V. V., Vergolуas, M. R. (2012). Pat. 97199 МРК: G01N 33/18. Sposib pidgotovky biologichnogo ob'jekta dlja vyznachennja toksychnosti vody. a201012982; zajavl. 01.11.2010; opubl. 10.01.2012; bjul. № 1.
Boltina, I. V., Vergolуas, M. R., Povjakelj, L. I., Zlacjkyj, I. A., Zavaljna, V. V., Kovalenko, O. V., Makarov, O. O., Zajecj, Je. R., Semenova, A. Ju. (2012). Materialy IX zj'ida ukrajinskogo tovarystva genetykiv selekcioneriv im. M. I. Vavylova. Kyiv: LOGOS, 4, 249–250.
Albertini, R. J., Anderson, D., Douglas, G. R., Hagmar, L., Hemminki, K., Merlo, F. et. al (2000). IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutation Research/Reviews in Mutation Research, 463 (2), 111–172. doi: 10.1016/s1383-5742(00)00049-1
Pavanello, S., Clonfero, E. (2000). Biological indicators of genotoxic risk and metabolic polymorphisms. Mutation Research/Reviews in Mutation Research, 463 (3), 285–308. doi: 10.1016/s1383-5742(00)00051-x
Goncharuk, V. V., Vergolуas, M. R. (2013). DSTU 7387:2013. Jakistj vody. Metod vyznachennja cyto- ta ghenotoksychnosti vody i vodnykh rozchyniv na klitynakh krovi prisnovodnoji ryby Danio rerio (Brachydanio rerio Hamilton-Buchanan).
Vergolyas, M. R., Lutsenko, T. V., Goncharuk, V. V. (2013). Cytotoxic effect of chlorophenols on cells of the root meristem of welsh onion (Allium fistulosum L.) seeds. Cytology and Genetics, 47 (1), 34–38. doi: 10.3103/s0095452713010118
Boltina, I. V. (2008). A. s. (Svidoctvo pro derzhavnu rejestraciju prav avtora na tvir) Modyfikacija metodu vyvchennja mutagennoi' aktyvnosti rechovyn (metafaznogo analizu aberacij hromosomv kul'turi limfocytiv peryferychnoi' krovi ljudyny) in vitro z metabolichnoju aktyvacijeju. № 23794; zajav. 21.12.2007; opubl. 05.03.2008.
Trakhtenbergh, Y. M., Tymofyevskaja, L. A., Kvjatkovskaja, Y. Ja. (1987). Metody yzuchenyja khronycheskogho dejstvyja khymycheskykh y byologhycheskykh zaghrjaznytelej. Riga: Zynatys, 172.
Derzhavni sanitarni normy ta pravyla. Ghighijenichni vymoghy do vody pytnoji, pryznachenoji dlja spozhyvannja ljudynoju (DSanPiN 2.2.4-171-10).
Balakhovskyj, Y. S.; Menjshykov, V. V. (Ed.) (1982). Rukovodstvo po klynycheskoj laboratornoj dyaghnostyke. Moscow: Medycyna, 235.
Antomonov, M. Ju. (2006). Matematycheskaja obrabotka y analyz medyko-byologhycheskykh dannykh. Kyiv: FMD, 558.
Goncharuk, V. V., Vergoljas, M. R., Boltyna, Y. V. (2013). Yssledovanye mutagennosty y genotoksychnosty pyt'evoj vodyy. Hymyja y tehnologyja vodyy, 35 (5), 426–435.
Fiskesjö, G. (2008). The Allium test as a standard in environmental monitoring. Hereditas, 102 (1), 99–112. doi: 10.1111/j.1601-5223.1985.tb00471.x
Goncharuk, V. V., Vergoljas, M. R. (2014). Toksycheskoe vlyjanye bakteryj Escherichia coli v zavysymosty ot yh soderzhanyja v vode na test-organyzmyy. Hymyja y tehnologyja vodyy, 36 (1), 83–91.
Goncharuk, V. V., Boltina, I. V., Vergolyas, M. R. (2011). Pat. 93964 МPК: G01N 33/18. Sposib vyznachennja mutagennoi' aktyvnosti pytnoi' vody i kul'tural'ne seredovyshhe dlja jogo realizacii'. a201000606; zajavl. 22.01.2010; jpubl. 25.03.2011; Bjul. № 6.
Ghoncharuk, V. V., Vergolyas, M. R., Boltina, I. V. (2011). Pat. 95717 MPK G 01 33/18. Sposib vyznachennja genotoksychnosti vodnogo seredovyshha. a201004569; zajavl. 19.04.2010; opubl. 25.08.2011.; Bjul. № 16.
Inoue, A., Yokomori, K., Tanabe, H., Mizusawa, H., Sofuni, T., Hayashi, Y. et. al (1997). Extensive genetic heterogeneity in the neuroblastoma cell line NB(TU)1. International Journal of Cance, 76 (6), 1070–1077. doi: 10.1002/(sici)1097-0215(19970917)72:6<1070::aid-ijc23>3.0.co;2-7
Arhipchuk, V. V., Goncharuk, V. V. (2001). Biotestirovanie kachestva vody na kletochnom urovne. Himija i tehnologija vody, 23 (5), 531–544.
Postel, S. (1987). State of the world. New York: W.W. Norton, 169–173.
Copyright (c) 2016 Maya Vergolyas
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.