OBTAINING AND CHARACTERISTIC OF THE AUTOLYSATE OF LACTIC ACID BACTERIA

Antonina Kapustian, Natalia Cherno

Abstract


Fragments of bacterial peptidoglycan relating to compounds of the muropeptide series have high biological activity. They have an immunological effect, due to the fact that they are signals for immune receptors and, accordingly, their activators. In order to obtain fragments of peptidoglycans, the autolysis of the bacterial mass Lactobacillus delbrueckii subsp. Bulgaricus 2–11 was performed and the characteristics of protein nature products of autolysate were given. To obtain cell suspensions at different growth stages, a growth curve of the bacterial culture was constructed for 48 hours. The autolysis of biomass was carried out at the end of the logarithmic phase of growth at the temperatures 37–90 0C and after the stationary phase of growth at the temperatures 37–90 0C for 7 days. The degree of autolytic changes was evaluated by the definition of colony forming units, the accumulation of amino acids, low molecular weight peptides and soluble protein in the autolysate. The results of studies of autolytic biomass changes have shown that the largest accumulation of amino acids occurs in autolysate, which was obtained at the end of the logarithmic phase of the growth of bacterial mass at exposures at 90 0C. The molecular-mass composition of protein nature compounds of the autolysate soluble fraction is determined. It is established that peptides with a molecular weight in the range corresponding to the molecular weight of the muropeptides are presented in the autolystate, but their quantity is rather insignificant.


Keywords


lactic acid bacteria; bacteriocins; autolysis; autolysate; peptidoglycan; muropeptide

Full Text:

PDF

References


Chapot-Chartier, M.-P., Kulakauskas, S. (2014). Cell wall structure and function in lactic acid bacteria. Microbial Cell Factories, 13, 9. doi: 10.1186/1475-2859-13-s1-s9

Glushanova, N. A. (2003). Biologicheskie svoystva laktobatsill. Bulletin of Siberian Medicine, 4, 50–58.

Moreira, L. O., Zamboni, D. S. (2012). NOD1 and NOD2 Signaling in Infection and Inflammation. Frontiers in Immunology, 3. doi: 10.3389/fimmu.2012.00328

Kawai, T., Akira, S. (2010). The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology, 11 (5), 373–384. doi: 10.1038/ni.1863

Harris, G. (Ed.) (2008). How the Immune System Recognizes Self and Nonself. Immunoreceptors and Their Signaling. Dordresht: Springer, 251. doi: 10.1007/978-4-431-73884-8

Shaphaev, E. G., Tsyirenov, V. Zh., Chebunina, E. I. (2015). Dezintegratsiya kletok v biotehnologii. Ulan-Ude, 96.

Martinez-Cuesta, M. C., Kok, J., Herranz, E., Pelaez, C., Requena, T., Buist, G. (2000). Requirement of Autolytic Activity for Bacteriocin-Induced Lysis. Applied and Environmental Microbiology, 66 (8), 3174–3179. doi: 10.1128/aem.66.8.3174-3179.2000

Kapustyan, A. I., Cherno, N. K. (2015). Prospects for using bioactive bacterial hydrolysates for nutritional supplementation of people with immune system disorders. Journal of Food Science and Technology, 9 (2 (31)), 18–25. doi: 10.15673/2073-8684.31/2015.44263

Cherno, N., Kapustyan, A. (2016). Immunological properties of the bacterial origin compounds. Food science and technology, 10 (3), 19–28. doi: 10.15673/fst.v10i3.175

Simova, E., Beshkova, D. (2007). Effect of growth phase and growth medium on peptidase activities of starter lactic acid bacteria. Le Lait, 87 (6), 555–573. doi: 10.1051/lait:2007036

Wood, B. J., ‎Warner, P. J. (Ed.) (2012). Genetics of Lactic Acid Bacteria. Berlin: Springer, 394.

Semak, I. V., Zyryanova, T. N., Gubich, O. I. (2007). Biohimiya belkov. Minsk: BSU, 49.

Gavrilin, M. V., Sen'chukova, G. V., Senchenko, S. P. (2007). Vybor optmal'nyh uslovii polucheniya gidrolizatov molochnokislyh bakterii termokislotnym sposobom. Himiko-farmatsevticheskiy zhurnal, 41 (2), 54–56.

MMG 233 2013 Genetics & Genomics Wiki. Available at: http://mmg-233-2013-genetics-genomics.wikia.com/wiki/File: Ion-exchange_column.jpg

Striegel, A. M., Yau, W. W., Kirkland, J. J., Bly, D. D. (2009). Development and detection of size separation by SEC. Modern Size-Exclusion Liquid Chromatography – Practice of Gel Permeation and Gel Filtration Chromatography. doi: 10.1002/9780470442876




DOI: http://dx.doi.org/10.21303/2504-5695.2018.00558

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Antonina Kapustian, Natalia Cherno

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

ISSN 2504-5695 (Online), ISSN 2504-5687 (Print)