DEVELOPMENT OF BIOTECHNOLOGY OF GETTING SELENIUM NANOSTRUCTURES WITH LACTOBACILLUS ACIDOPHILUS CULTURE

Leonid Kaprellyanz, Natalia Zykovа, Arsen Petrosyants, Alexander Zykov

Abstract


Selenium nanostructures are characterized with high activity and antibacterial properties. Selenium nanoparticles, received by the biotechnological method using microorganisms are not toxic and free from admixtures of other toxic elements. For getting selenium nanostructures, there was set a problem of development of the biotransfrormation technology of inorganic selenium by the culture Lactobacillus acdophilus, strain 412/307 from the museum of the Department of Biochemistry, Microbiology and Physiology of Nutrition Odessa National Academy of Food Technologies (Ukraine). Selenium nanostructures are the side product in the process of selenocysteine formation. There was realized the study of the influence of the sodium selenite concentration on the growth speed and generation duration of lactobacteria on MRS medium for determining the main technological parameters. There was determined the quantitative content of accumulated selenium at different initial concentrations of sodium selenite in the medium. The important part of the elaborated technology is destruction of cellular walls of microorganisms for removal selenium nanoparticles. There was studied the influence of different physical-chemical factors of cells’ destruction. There was offered to use the combined influence of saline acid, freezing and microwave field for destructing cellular walls of lactobacteria biomasses. There is presented the scheme of the technology of getting “Nanoselenium” and given physical-chemical parameters of the received product.


Keywords


sodium selenite; lactobacteria; nanostructures; optic density; specific speed of growth; generation duration

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DOI: http://dx.doi.org/10.21303/2504-5695.2018.00560

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Copyright (c) 2018 Leonid Kaprellyanz, Natalia Zykovа, Arsen Petrosyants, Alexander Zykov

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ISSN 2504-5695 (Online), ISSN 2504-5687 (Print)