DEVELOPMENT OF BIOTECHNOLOGY OF GETTING SELENIUM NANOSTRUCTURES WITH LACTOBACILLUS ACIDOPHILUS CULTURE
Abstract
Selenium nanostructures are characterized with high activity and antibacterial properties. Selenium nanoparticles, received by the biotechnological method using microorganisms are not toxic and free from admixtures of other toxic elements. For getting selenium nanostructures, there was set a problem of development of the biotransfrormation technology of inorganic selenium by the culture Lactobacillus acdophilus, strain 412/307 from the museum of the Department of Biochemistry, Microbiology and Physiology of Nutrition Odessa National Academy of Food Technologies (Ukraine). Selenium nanostructures are the side product in the process of selenocysteine formation. There was realized the study of the influence of the sodium selenite concentration on the growth speed and generation duration of lactobacteria on MRS medium for determining the main technological parameters. There was determined the quantitative content of accumulated selenium at different initial concentrations of sodium selenite in the medium. The important part of the elaborated technology is destruction of cellular walls of microorganisms for removal selenium nanoparticles. There was studied the influence of different physical-chemical factors of cells’ destruction. There was offered to use the combined influence of saline acid, freezing and microwave field for destructing cellular walls of lactobacteria biomasses. There is presented the scheme of the technology of getting “Nanoselenium” and given physical-chemical parameters of the received product.
Downloads
References
Shin, Т. (2001). Selenium in the human organism. Journal of microbiolgy and biotechnology, 11, 97–105.
Radhika, R. S., Gayathri, S. (2015). Extracellular biosynthesis of Selenium nanoparticles using some species of Lactobacillus. Indian Journal of Geo-Marine Sciences, 43 (5), 766–775.
Oremland, R. S., Herbel, M. J., Blum, J. S., Langley, S., Beveridge, T. J., Ajayan, P. M. et. al. (2004). Structural and Spectral Features of Selenium Nanospheres Produced by Se-Respiring Bacteria. Applied and Environmental Microbiology, 70 (1), 52–60. doi: 10.1128/aem.70.1.52-60.2004
Fesharaki, P. J., Nazari, P., Shakibaie, M., Rezaie, S., Banoee, M., Abdollahi, M., Shahverdi, A. R. (2010). Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process. Brazilian Journal of Microbiology, 41 (2), 461–466. doi: 10.1590/s1517-83822010000200028
Sasidharan, S., Balakrishnaraja, R. (2014). Comparison Studies on the Synthesis of Selenium Nanoparticles by Various Microorganisms. International Journal of Pure & Applied Bioscience, 2 (1), 112–117.
Prokisch, J. (2010). Process for Producing Elemental Selenium Nanospheres. United States, 634.
Egorova, E. A. (2006). Poluchenie i eksperimental'naya otsenka novykh pishhevykh istochnikov organicheskikh form selena. Moscow, 121.
Visha, P., Nanjappan, K., Selvaraj, P., Jayachandran, S., Elango, A., Kumaresan, G. (2015). Biosynthesis and Structural Characteristics of Selenium Nanoparticles using Lactobacillus Acidophilus Bacteria by Wet Sterilization Process. International Journal of Advanced Veterinary Science and Technology, 4 (1), 178–183. doi: 10.23953/cloud.ijavst.183
Nidhi, S. (2014). Biosynthesis of silver and selenium nanoparticles by Bacillus sp. JAPSK2 and evaluation of antimicrobial activity. Applied and environmental microbiology, 3, 135–141.
Sonam, M. (2014). A superficial synthesis of selenium nanospheres using wet chemical approach. International Journal of Nanotechnology and Application, 3, 7–13.
Pankov, A. R., Goryainova, E. R., Zhernosek, A. I. (2013). Statisticheskie metody obrabotki dannykh. Moscow, 212.
Copyright (c) 2018 Leonid Kaprellyanz, Natalia Zykovа, Arsen Petrosyants, Alexander Zykov
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.