INDICES OF FATTY ACIDS SPECTRUM OF LIPIDS IN THE BLOOD SERUM OF STERLET OF DIFFERENT AGE

Roza Suleimanova, Dmytro Melnychuk, Liliia Kalachniuk

Abstract


As the only sturgeon living in freshwater, sterlet is important for breeding in industrial fish farms, where lately cases of early mortality of this species have been detected. Hence, in order to preserve the species, it is important to study the parameters of fatty acid (FA) composition of lipid fractions of the blood serum of sterlet.

Here we present changes of fatty acid composition in different lipid fractions of the blood serum of sterlets of different age (namely two-, three- and nine-year-old) with the masses 0.3–0.4, 0.5–0.6 and 5–6 kgfor the age-groups of fish, respectively. Fatty acid (FA) composition was determined using gas-chromatography on HRGC 5300 (Italy) in Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU). Fatty acid composition of starlet blood serum is presented by saturated and unsaturated high-molecular weight carboxylic acids, mostly palmic, stearic, oleic and linoleic. In the phospholipids fraction, there was a moderate increase in saturated and monounsaturated fatty acids and a slight decrease in polyunsaturated fatty acids depending on the age of fish. As for free fatty acids, there was a drop in the saturated ones depending on the starlet age. Among the free fatty acids of sterlet blood serum, we identified 28 acids, of them 39 %, 35 % and 30 % were saturated in 2-, 3-year-old and mature fish, respectively. Monounsaturated FA content was 14 %, 23 % and 23 % in 2-, 3-year-old and adult sterlet fish, and polyunsaturated FA content –46 %, 41 % and 36 %, respectively. The data can be used for the theoretical verification of correcting supplementary feed and premixes.


Keywords


sterlet; blood; lipids; saturated fatty acids; monounsaturated fatty acids; polyunsaturated fatty acids

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References


Suleimanova, R. R., Hudz, E. А., Melnychuk, D. О., Kalachniuk, L. H. (2017). Age-related changes phospholipids of sterlet in liver and dorsal muscles. The Ukrainian Biochemical Journal, 89 (1), 71–75. doi: 10.15407/ubj89.01.071

Jastroch, M., Giroud, S., Barrett, P., Geiser, F., Heldmaier, G., Herwig, A. (2016). Seasonal Control of Mammalian Energy Balance: Recent Advances in the Understanding of Daily Torpor and Hibernation. Journal of Neuroendocrinology, 28 (11). doi: 10.1111/jne.12437

Yanovych, N. (2013). Fatty acids composition of carp (cyprinus carpio) skeletal muscles under influence of different copper and zinc concentration in water. Ribogospodarsʹka Nauka Ukraïni, 2 (24), 70–75. doi: 10.15407/fsu2013.02.070

Suleimanova, R. R., Hudz, I. A., Melnychuk, D. O., Kalachniuk, L. H. (2017). Age peculiarities of the content of phospholipids in the blood of sterlet. Reports of the National Academy of Sciences of Ukraine, 5, 98–101. doi: 10.15407/dopovidi2017.05.098

Folch, J., Lees, M., Sloane Stanley, C. (1957). A simple method for the isolation and purification of total lipides from animal tissues. Ukrainian Biochemical Journal, 226 (1), 497–511.

Carreau, J. P., Dubacq, J. P. (1978). Adaptation of a macro-scale method to the micro-scale for fatty acid methyl transesterification of biological lipid extracts. Journal of Chromatography A, 151 (3), 384–390. doi: 10.1016/s0021-9673(00)88356-9

Reznikov, O. H. (2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh. Pershyi natsionalnyi konhres z bioetyky [General ethical principles of animal experimentation. First National Congress on Bioethics]. Endocrinol., 8 (1), 142–145.

Tsvetkova, M. V., Khirmanov, V. N., Zybina, N. N. (2010). Significance of non–etherificated fatty acids in pathogenesis of cardiovascular diseases, Arterial’naya Gipertenziya, 6 (1), 93–103.

Khuda, L. V., Marchenko, M. M., Khudyi, O. I. (2014). Zhyrnokyslotnyi sklad m’iaziv sterliadi, vyroshchenoi v umovakh rybovodnoi retsyrkuliatsiinoi systemy [Fatty acid composition of muscles of sterlet, grown in the conditions of a fish water recirculation system]. Ukrainian Biochemical Journal, 86, 263.

Govorin, A. V. (2010). Non-coronarogenic myocardial damages, Novosibirsk: Nаuka, 230.

Kuo, C. M., Hsieh, S. L. (2006). Comparisons of physiological and biochemical responses between milkfish (Chanos chanos) and grass carp (Ctenopharyngodon idella) to cold shock. Aquaculture, 251 (2-4), 525–536. doi: 10.1016/j.aquaculture.2005.05.044

Lyavrin, B. Z., Kurant, V. Z., Khomenchuk, V. O., Grubinko, V. V. (2014). Species peculiarities of the lipid composition of some freshwater fish tissues of the Western Podillya. Reports of the National Academy of Sciences of Ukraine, 8, 123–127. doi: 10.15407/dopovidi2014.08.123

Nelson, D. L., Cox, M. M. (2017). Lehninger Principles of Biochemistry. New York: W.H. Freeman, 1328.

Melnychuk, D. O., Melnychuk, S. D., Arnauta, O. V. (2004). Influence of carbon dioxide on the environment preservation of red blood cells in stored blood of animals. Scientific Bulletin of NAU, 75, 163–165.

Yli-Jama, P., Seljeflot, I., Meyer, H. E., Hjerkinn, E. M., Arnesen, H., Pedersen, J. I. (2002). Serum non-esterified very long-chain PUFA are associated with markers of endothelial dysfunction. Atherosclerosis, 164 (2), 275–281. doi: 10.1016/s0021-9150(02)00067-9

Leeson, C. (2002). Relationship between circulating n-3 fatty acid concentrations and endothelial function in early adulthood. European Heart Journal, 23 (3), 216–222. doi: 10.1053/euhj.2001.2728

Silkin, Y. A., Silkina, E. N., Zabelinskii, S. A. (2012). Peculiarities of the phospholipid and fatty acid composition of erythrocyte plasma membranes of the black sea fish. Journal of Evolutionary Biochemistry and Physiology, 48 (1), 43–51. doi: 10.1134/s0022093012010069

Gritsyanak, І. І., Smolyaninov, K. B., Janovich, V. G. (2010). Obmin lipidiv u ryb [Exchange of lipids in fish]. Lviv: Triad plus.

Li, G., Sinclair, A. J., Li, D. (2011). Comparison of Lipid Content and Fatty Acid Composition in the Edible Meat of Wild and Cultured Freshwater and Marine Fish and Shrimps from China. Journal of Agricultural and Food Chemistry, 59 (5), 1871–1881. doi: 10.1021/jf104154q

Du, Z.-Y., Clouet, P., Zheng, W.-H., Degrace, P., Tian, L.-X., Liu, Y.-J. (2006). Biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (Ctenopharyngodon idella) fed high-fat diets. British Journal of Nutrition, 95 (5), 905–915. doi: 10.1079/bjn20061733




DOI: http://dx.doi.org/10.21303/2504-5695.2018.00578

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