Victoria Kasianchuk, Oleksanda Berhilevych, Inna Negai, Lubov Dimitrijevich, Tatyana Marenkova


The aim of the research was to determine features of the elemental composition of polyfloral honey from the Odessa region (Ukraine) by the method of x-ray fluorescence for using these features in the geographic marking of the region of honey origin. A choice of honey from the Odessa region is explained by its relatively good ecology, optimal maritime climate and enough motley grass resources for gathering nectar by bees. At the same time the Odessa region occupies the fourth place among regions – honey producers in Ukraine with a right to export honey beyond the country with specific weight 10 % from the total export of this product.

11 samples of fresh honey and 14 samples of honey, stored during one year were studied as to the content of 22 mineral elements. The elemental analysis of honey was realized on the energy-dispersive x-ray fluorescent spectrometer ElvaX Light SDD. Each sample was studied for 5 times. The obtained results were statistically processed by the standard methodology. The determination distinctness of mass shares of metals is no worse than 0,1 %. Limits of finding admixtures of heavy metals in the light matrix are no worse than 1 ppm. The studies were realized during 2016-2017.

It has been established, that lyophilization of honey doesn’t essentially influence determination parameters of the elemental composition of honey by the method of x-ray fluorescence. The analysis of energy-dispersive spectrums of mineral elements determined that marker elements for honey from the Odessa region are Cl, K, Ca, that prevail among other studied mineral elements. There has been established the typical intensity of a signal of energy-dispersive spectrums for Cl, K, Ca of honey from the Odessa region that correspond to the following values: for fresh honey Cl from 27075 to 29429; K from 47 296 to 41 546; Ca from 7572 to 6928; for honey, stored during one year: Cl from 40383 to 37044; K from 43 589 to 42 591;Ca from 15495 to 10006. These parameters may serve as markers for honey from the Odessa region. At the same time the obtained results may be used for controlling the quality of natural honey by its element composition for identifying a geographic place of origin.


polyfloral honey; elemental analysis; energy-dispersion; X-ray fluorescent spectrometer; Cl; K; Ca

Full Text:



García, N. L. (2018). The Current Situation on the International Honey Market. Bee World, 95 (3), 89–94. doi:

Machado De-Melo, A. A., Almeida-Muradian, L. B. de, Sancho, M. T., Pascual-Maté, A. (2017). Composition and properties of Apis mellifera honey: A review. Journal of Apicultural Research, 57 (1), 5–37. doi:

Serrano, S., Villarejo, M., Espejo, R., Jodral, M. (2004). Chemical and physical parameters of Andalusian honey: classification of Citrus and Eucalyptus honeys by discriminant analysis. Food Chemistry, 87 (4), 619–625. doi:

Mulugeta, E. (2017). Physicochemical Characterization and Pesticide Residue Analysis of Honey Produced in West Shewa Zone, Oromia Region, Ethiopia. American Journal of Applied Chemistry, 5 (6), 101–109. doi:

Lenhardt, L., Zeković, I., Dramićanin, T., Dramićanin, M. D., Bro, R. (2014). Determination of the Botanical Origin of Honey by Front-Face Synchronous Fluorescence Spectroscopy. Applied Spectroscopy, 68 (5), 557–563. doi:

Thrasyvoulou, A., Tananaki, C., Goras, G., Karazafiris, E., Dimou, M., Liolios, V. et. al. (2018). Legislation of honey criteria and standards. Journal of Apicultural Research, 57 (1), 88–96. doi:

Uršulin-Trstenjak, N., Levanić, D., Šušnić, S., Lasić, D., Šušnić, V. (2015). Determination of geographical origin of black locust honey of five Croatian regions by applying the PCA method. Journal of Hygienic Engineering and Design, 13, 68–74.

Uršulin-Trstenjak, N., Levanić, D., Primorac, L., Bošnir, J., Vahčić, N., Šarić, G. (2016). Mineral profile of Croatian honey and differences due to its geographical origin. Czech Journal of Food Sciences, 33 (2), 156–164. doi:

Silva, L. R., Sousa, A., Taveira, M. (2017). Characterization of Portuguese honey from Castelo Branco region according to their pollen spectrum, physicochemical characteristics and mineral contents. Journal of Food Science and Technology, 54 (8), 2551–2561. doi:

Chen, H., Fan, C. L., Chang, Q. Y., Pang, G. F., Cao, Y. F., Jin, L. H., Hu, X. Y. (2015). Application of ICP-MS method in the determination of mineral elements in vitex honey for the classification of their geographical origins with chemometric approach. Spectroscopy and spectral analysis, 35 (1), 212–216.

Ribeiro, R. de O. R., Mársico, E. T., de Jesus, E. F. O., da Silva Carneiro, C., Júnior, C. A. C., de Almeida, E., Filho, V. F. do N. (2014). Determination of Trace Elements in Honey from Different Regions in Rio de Janeiro State (Brazil) by Total Reflection X-Ray Fluorescence. Journal of Food Science, 79 (4), T738–T742. doi:

Zhou, J., Suo, Z., Zhao, P., Cheng, N., Gao, H., Zhao, J., Cao, W. (2013). Jujube Honey from China: Physicochemical Characteristics and Mineral Contents. Journal of Food Science, 78 (3), C387–C394. doi:

Kılıç Altun, S., Dinç, H., Paksoy, N., Temamoğulları, F. K., Savrunlu, M. (2017). Analyses of Mineral Content and Heavy Metal of Honey Samples from South and East Region of Turkey by Using ICP-MS. International Journal of Analytical Chemistry, 2017, 1–6. doi:

Zalog zdorov'ya. Ekologicheskiy reyting oblastey Ukrainy. Available at:

Borovikov, V. (2003). Statistica. Iskusstvo analiza dannyh na komp'yutere. Sankt-Peterburg: Piter, 688.



  • There are currently no refbacks.

Copyright (c) 2019 Victoria Kasianchuk, Oleksanda Berhilevych, Inna Negai, Lubov Dimitrijevich, Tatyana Marenkova

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

ISSN 2504-5695 (Online), ISSN 2504-5687 (Print)