THE INFLUENCE OF RATS-MOTHERS FOOD RATION WITH NUTRIENT DEFICIENCY ON THE STRUCTURAL-FUNCTIONAL STATE OF THE LIVER OF THEIR TWO MONTHS OFFSPRING

Milena Kuznetsova

Abstract


The aim of this study was to determine the influence of rats-mothers food ration with nutrient deficiency on the morphology and functional status of the liver of their two months offspring.

Materials and methods. The experiments were performed on the offspring of WAG population rats, which was prenatal under nutritional deficiency. The offspring of rats (26 specimens) were divided into two groups, 50 % of the rats constituted the control group (i.e., their mothers received the vivarium baseline diet), the other 13 animals were in the 2nd (main) group. The young rats were removed from the experiment two months after birth by decapitation. Morpho-functional condition of the liver of the offspring rats treated with pregnancy deficiency of fat and carbohydrate diet was evaluated according to the results of immunohistochemical study of markers of liver vascular endothelium damage - endothelial synthase of nitric oxide (eNOS) and inducible nitric oxide synthase (iNOS) and biochemical study of liver tissue homogenates (studied fractional composition of lipids and organ glycogen).

Results. It was found that the nutritional deficiency of rats-mothers had a significant effect on the structural and functional state of the liver of their bi-monthly offspring, which was manifested by a decrease in the level of endothelial synthase expression, which indicated a high degree of damage to the liver endothelium. Biochemical study of liver homogenates revealed signs of impaired secretion and reuptake of lipids, which were manifested by an increase in CL, TG and EFAs accumulation, as well as a decrease in liver PL and glycogen, which indicated the presence of pronounced disorders of carbohydrate and lipid metabolism.

Conclusions. The obtained data allow us to establish the negative nature of the effect of deficiency of fats and carbohydrates in the diet of rats-mothers on the structural and functional state of the liver of their bi-monthly offspring. This condition was accompanied, both by signs of liver vascular endothelial damage and metabolic disorders, this all suggests that the identified disorders may be a risk factor for fatty hepatosis as well as liver cirrhosis in the future.


Keywords


liver; offspring; morphology; immunohistochemistry; homogenate; fat and carbohydrate deficiency diet; pregnancy

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DOI: http://dx.doi.org/10.21303/2585-663.2019.001093

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ISSN 2585-6634 (Online), ISSN 2585-6626 (Print)