MICROBIOLOGICAL STUDIES OF ANTI-HISTAMINE SUPPOSITORIES "LORAVIT" FOR CHILDREN

Iryna Herasymova, Aleksander Tikhonov

Abstract


Among the current problems of medicine and pharmacy, attracting the attention of researchers around the world, allergic diseases are on an important place; for example, bronchial asthma, allergic rhinitis, atopic dermatitis, and others. These pathologies affect their abundance not only among adults, but also among children [1]. According to statistics, almost every child had allergic reactions to drugs, food, clothing, hygiene products, etc. at least once [2]. In this regard, we have developed a new anti-allergic drug for children in the form of suppositories under the conditional name "Loravit".

The purpose of this work was to conduct microbiological research of new anti-allergic drug "Loravit" for children in the rectal dosage form.

During researches, the correlation of the growth properties of nutrients was analyzed and the microbiological purity of the drug "Loravit" was studied. A thioglyсol semi-liquid substratum, Saburo’s substratum, solid nutrient substrata: nutrient agar, Saburo’s substratum, Chistovich's substratum, nutrient agar based on blood agar, Endo’s substratum, and also test microorganisms - Staphylococcus aureus ATCC 6538, Esherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Bacillus subtilis ATCC 6633, Candida albicans ATCC 885/653 were used for carrying out these studies.

In the study of microbiological purity, the method of direct seeding on liquid nutrient substrata and the method of deep sowing were applied.

As a result of the conducted studies it was established that a new medicinal product for children in the form of suppositories for the treatment of allergic diseases meets the requirements of the State Pharmacopoeia of Ukraine and European Pharmacopeia for the indicator "Microbiological purity". The norms of microbiological purity of suppositories "Loravit" are also established. The test results were included in the draft quality control methods for the developed drug.


Keywords


Allergic diseases; children; suppositories; loratadine hydrochloride; microbiological purity; deep sowing; direct sowing

Full Text:

PDF

References


Ogiev, V. A. (2015). Epidemiologiia astmy i allergii u detei [Epidemiology of asthma and allergies in children]. Kharkiv: Shchedra sadyba plius, 336.

Rona, R. J., Keil, T., Summers, C., Gislason, D., Zuidmeer, L., Sodergren, E. et. al. (2007). The prevalence of food allergy: A meta-analysis. Journal of Allergy and Clinical Immunology, 120 (3), 638–646. doi: 10.1016/j.jaci.2007.05.026

Rao, M. (2003). Occupational rhinitis and asthma. EAACI Newsletter, 6, 70–81.

Pukhlyk, B. M., Dytiatkivska, Ie. M., Hohunska, I. V. (2012). Pytannia poshyrenosti ta ekonomichnoi efektyvnosti likuvannia alerhiinykh zakhvoriuvan orhaniv dykhannia v Ukraini [Issues of the prevalence and economic efficiency of treatment of allergic diseases of the respiratory organs in Ukraine]. Clinical immunology. Allergology. Infectology, 2, 5–7.

Balabolkin, I. I. (2013). Food allergy in children: modern aspects of pathogenesis and approaches to therapy and prophylaxis. Immunopathology, allergology, infectology, 3, 36–46.

Kozulina, I. E., Kurbacheva, O. M., Ilina, N. I. (2014). Allergiia segodnia. Analiz novykh epidemiologicheskikh dannykh [Analysis of new epidemiological data]. Russian Allergology Journal, 3, 3–10.

Branum, A. M., Lukacs, S. L. (2009). Food Allergy Among Children in the United States. Pediatrics, 124 (6), 1549–1555. doi: 10.1542/peds.2009-1210

Morais-Almeida, M., Santos, N., Pereira, A. M., Branco-Ferreira, M., Nunes, C., Bousquet, J., Fonseca, J. A. (2013). Prevalence and classification of rhinitis in preschool children in Portugal: a nationwide study. Allergy, 68 (10), 1278–1288. doi: 10.1111/all.12221

Gatckaia, D. O., Koritckaia, I. V. (2015). Dinamika rasprostranennosti allergicheskikh zabolevanii sredi detei i molodezhi Vinnitckoi oblasti (rezultaty 1-go etapa epidemiologicheskogo issledovaniia) [The dynamics of the prevalence of allergic diseases among children and youth in the Vinnytsia region (the results of the first stage of epidemiological research)]. Asthma and allergies, 1, 17–22.

Cingi, C., Songu, M., Ural, A., Annesi-Maesano, I., Erdogmus, N., Bal, C. et. al. (2011). The Score For Allergic Rhinitis study in Turkey. American Journal of Rhinology and Allergy, 25 (5), 333–337. doi: 10.2500/ajra.2011.25.3665

Johannes, R. (2007). Allergy is a Global Problem. ACII J. WAO, 12 (2), 26.

Troshina, A. A., Dolgushin, I. I., Dolgushina, V. F., Bakhareva, L. I., Kurnosenko, I. V., Nadvikova, T. V. (2015). Mikrobiologicheskaia effektivnost preparata na osnove gialuronidazy u patcientok s khronicheskim endometritom i miomoi matki [Microbiological efficacy of the drug based on hyaluronidase in patients with chronic endometritis and the myoma of the uterus]. Gynecology, 17 (6), 10–12.

Slivkin, D. A., Suslina, S. N., Filonova, E. V., Slivkin, A. I., Belenova, A. S., Veretennikova, M. A. (2012). Izuchenie mikrobiologicheskoi chistoty i stabilnosti novykh tabletirovannykh lekarstvennykh form nootropnogo deistviia [The study of the microbiological purity and stability of new tableted medicinal forms with nootropic action]. Bulletin of Voronezh State University. Series: chemistry, biology, pharmacy, 1, 164–167.




DOI: http://dx.doi.org/10.21303/2585-663.2017.00446

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Iryna Herasymova, Aleksander Tikhonov

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

ISSN 2585-6634 (Online), ISSN 2585-6626 (Print)