CONTENT OF DIENE KOH'UGATIVES AND MALONIC DIALDEHYDE IN BLOOD FOR RATS IN DYNAMICS OF FORMATION OF IMMOBILIZATIONAL STRESS

Oksana Lys

Abstract


Nowadays, one of the most important problems are stressful reaction of human and animal systems.

The purpose of our study was to find out the peculiarities of the dynamics of changes in the indices of diene conjugates and malonic dialdehyde in blood in rats under the conditions of development of immobilization stress.

Material and methods of research. The experiments were carried out on white male rats of the Vistar line weighing 180–200 g which were divided into 3 groups for 10 animals (one control and three tested). The control group of rats was injected with 1 mg physiological saline per kg body weight intraperitoneally. The animals were decapitated under anes the siaca used by sodium thiopental (intraperitoneal injection of 1 % solution of 50 mg / kg) and withdrawn from the experiment after 1, 3, and 5 days. In blood plasma was determined the content of diene conjugates and malonic dialdehyde in stress-induced rats, the groups were compared with the control group rats.

Results. The state of the processes (POFs) was assessed by the content of blood vessels detected in different periods (1 st, 3 rd, and 5 th day) after conducting of IS in rats. Under the conditions of the development of the IS, an increase in the content of DK was observed at 116.96 % (p<0.05) in the experimental group1 accordingly on the 1st day after the IS compared with the control. For the 3rd day of the study, the level of DK increased by 44.24 % (p<0.05) in experimental group 2, against the intact group of animals. Experimental group 3, in which animals were withdrawn from the IS for the 5th day, was characterized by an increase in the content of DK by 41.84 % (p<0.05) compared with the control group. Another indicator of MDA in the blood for 1 day of the experiment in experimental group 1 increased by 104.15 % (p<0.05) in the case of IS in relation to the intact group. On the 3rd day of the experiment under the conditions of development of IP, the level of MDA increased by 83.9 % (p<0.05), against control. At the 5th day of the experiment, MDA level was 30.1 % (p<0.05) for IS, when compared with control

Conclusion. Consequently, the experiment made it possible to establish the significant activation of the LP processes, which we determined with increasing levels of DC and MDA in animals under IS conditions, the most of it was on the 1th day of the experiment.


Keywords


rats; blood; lipid peroxidation; antioxidant defense; immobilization stress; diene conjugates; malonic dialdehyde

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DOI: http://dx.doi.org/10.21303/2585-663.2018.00751

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ISSN 2585-6634 (Online), ISSN 2585-6626 (Print)