PECULIARITIES OF HISTOMORPHOLOGICAL LIVER CHANGES ASSOCIATED WITH COMBINED EFFECT OF ACTIVE SUBSTANCES WITH ONE-PURPOSE ACTION

Sergii Omel’chuk, Alina Syrota

Abstract


Some studies have proven possible potentiating toxic effect of combined pesticides. The potentiating effect may be related to the impact of a certain substance on enzymatic activity of another one. Due to combined preparations popularity, the importance of evaluating probability of summation and synergetic or antagonizing action of the one-purpose substances becomes particularly important.

Aim of the research is to study peculiarities of histomorphological liver changes associated with combined effect of active substances with one-purpose action.

Methods. The authors have used toxicological, histochemical and statistical study methods. 45 rats have been used for the study. The liver specimens for histochemical analysis were selected immediately after dissection of the rats, from which the 5 micrometer sections were prepared, using freezing microtome. After leaving the sections in cold acetone, the histochemical reaction on determining the γ-glutamyl transpeptidase, a transformed hepatocyte marker, which forms nodules while proliferates, was conducted.

Results. The authors have established that the above-mentioned characteristics in animals which were administered the drug (benthiavalicarb-isopropyl+folpet) do not differ from negative control group. General specific area of nodules per сm2 and specific quantity of nodules per сm2 haven’t reliably changed, the control group exceeding these data by 18 % and 12 %, respectively.

Discussion. The study of the benthiavalicarb-isopropyl and folpet mixture effect on animals has not revealed liver tissue proliferation of the carcinogen-transformed cells and appearance of hyperplastic nodes, which express the γ-glutamyl transpeptidase, a histochemical marker of pre-tumor changes.

So, the fungicide (benthiavalicarb-isopropyl+folpet) has not induced multiplication or size increase of glutamyl transpeptidase nodules, which evidences about loss of the modifying effect of folpet on the benthiavalicarb-isopropyl carcinogenicity in this preparation formula.

The obtained results may be used when deciding on administration of combined fungicides with active substances of one-purpose action.


Keywords


fungicides; γ-glutamyl transpeptidase; benthiavalicarb-isopropyl; folpet; histochemical analysis; N-nitrosodimethylamine; N-Nitroso-N-Metylurea; N-nitrosobis (2-hydroxypropyl) amine; rats; promotor activity

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References


Stavnichenko, P. V., Antonenko, A. M., Bardov, V. G. (2017). Forecasting of development of acute poisonings in agricultural workers while using combined formulations based on difenoconazole. Medicni Perspektivi (Medical Perspectives), 22 (3), 116–121. doi: https://doi.org/10.26641/2307-0404.2017.3.111938

Leadbeater, A., Gisi, U. (2009). The Challenges of Chemical Control of Plant Diseases. Recent Developments in Management of Plant Diseases, 3–17. doi: https://doi.org/10.1007/978-1-4020-8804-9_1

Amr, S., Dawson, R., Saleh, D. A., Magder, L. S., St. George, D. M., El-Daly, M. et. al. (2014). Pesticides, Gene Polymorphisms, and Bladder Cancer Among Egyptian Agricultural Workers. Archives of Environmental & Occupational Health, 70 (1), 19–26. doi: https://doi.org/10.1080/19338244.2013.853646

Omidakhsh, N., Bunin, G. R., Ganguly, A., Ritz, B., Kennedy, N., von Ehrenstein, O. S. et. al. (2017). Parental occupational exposures and the risk of childhood sporadic retinoblastoma: a report from the Children’s Oncology Group. Occupational and Environmental Medicine, 75 (3), 205–211. doi: https://doi.org/10.1136/oemed-2017-104404

Depczynski, J. C. (2017). A population-based examination of cancer in New South Wales farm residents compared to rural non-farm and urban residents. University of Sydney. Available at: https://ses.library.usyd.edu.au/handle/2123/17114

Kim, K.-H., Kabir, E., Jahan, S. A. (2017). Exposure to pesticides and the associated human health effects. Science of The Total Environment, 575, 525–535. doi: https://doi.org/10.1016/j.scitotenv.2016.09.009

Benthiavalicarb-Isopropyl; pesticide tolerance (2006). Federal Register, 71 (170), 51998–52003. Available at: https://www.gpo.gov/fdsys/pkg/FR-2006-09-01/pdf/06-7313.pdf

De Albuquerque, N. C. P., Carrão, D. B., Habenschus, M. D., de Oliveira, A. R. M. (2018). Metabolism studies of chiral pesticides: A critical review. Journal of Pharmaceutical and Biomedical Analysis, 147, 89–109. doi: https://doi.org/10.1016/j.jpba.2017.08.011

Folpet; pesticide tolerance (2004). Federal Register, 69 (164), 52182–52192. Available at: https://www.gpo.gov/fdsys/pkg/FR-2004-08-25/pdf/04-19036.pdf

Ito, N., Shirai, T., Hasegawa, R.; Vainio, H., Magee, P. N., McGregor, D. B., McMichael, A. J. (Eds.) (1992). Medium term bioassays for carcinogens. Mechanism of carcinogenesis in riskidentification. The IARC Scientific Publications No. 93. Lyon: IARC, 353–388.

Sakthisekaran, D., Rajendran, P., Rengarajan, T., Nishigaki, I., Ekambaram, G. (2014). Potent chemopreventive effect of mangiferin on lung carcinogenesis in experimental Swiss albino mice. Journal of Cancer Research and Therapeutics, 10 (4), 1033. doi: https://doi.org/10.4103/0973-1482.137966

Wang, F., Zhu, Y., Zhou, L., Pan, L., Cui, Z., Fei, Q. et. al. (2015). Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific γ-Glutamyltranspeptidase. Angewandte Chemie International Edition, 54 (25), 7349–7353. doi: https://doi.org/10.1002/anie.201502899

McGlynn, K. A., Petrick, J. L., London, W. T. (2015). Global Epidemiology of Hepatocellular Carcinoma. Clinics in Liver Disease, 19 (2), 223–238. doi: https://doi.org/10.1016/j.cld.2015.01.001

Bagliy, Ye. A., Nedopitanska, N. M., Lisovska, V. S., Reshavska, O. V. (2011). Vplyv avermektyniv na iniciyovani nitrozodietylaminom gepatocyty shhuriv. Suchasni problemy toksykologiyi, 1, 22–26.

U.S. EPA (2017). Chemicals Evaluated for Carcinogenic Potential. Available: http://npic.orst.edu/chemicals_evaluated.pdf




DOI: http://dx.doi.org/10.21303/2585-663.2018.00765

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ISSN 2585-6634 (Online), ISSN 2585-6626 (Print)